RNA干扰XBP1基因表达对人胶质瘤细胞增殖及凋亡的影响

The effect of inhibition of XBP1 gene expression on human glioma cell proliferation and apoptosis

目的探讨X-盒结合蛋白1(X-box binding protein 1,XBP1)对人脑胶质瘤细胞增殖与凋亡的影响。方法设计并合成干扰XBP1表达的小干扰RNA(siXBP1),转染人脑胶质瘤U251细胞,MTT法检测细胞的增殖能力,流式细胞术检测细胞凋亡率,Hoechst 33258染色观察细胞凋亡,Western blot法检测Bax、Caspase-3及Bcl-2的表达水平。结果 siXBP1可有效抑制U251细胞增殖,促进细胞凋亡,Western blot显示,siXBP1上调了U251细胞中促凋亡信号分子Bax及Caspase-3的表达,抑制了U251细胞中抗凋亡信号分子Bcl-2的表达。结论下调脑胶质瘤细胞XBP1基因表达有望为脑胶质瘤的治疗提供理想的分子靶点。

Objective To study the effects of inhibition of X-box binding protein 1(XBP1) gene expression on human glioma cell proliferation and apoptosis.Methods Small interference RNA(siXBP1) was designed and synthesised to interference of XBP1 expression, transfection in human brain glioma U251 cells, MTT method was used to detect cell proliferation, flow cytometry was used to detect cell apoptosis rate, Hoechst 33258 dying was used to observe cell apoptosis, Western blot method was used to detect the expression levels of Bax,Caspase-3 and Bcl-2.Results siXBP1 could effectively inhibit U251 cell proliferation, promote U251 cell apoptosis, Western blot results showed that siXBP1 promoted the expression of promote apoptosis signaling molecules Bax and Caspase-3, suppressed the expression of anti-apoptosis signal molecule Bcl-2 in U251 cells.Conclusion The inhibition of XBP1 gene expression could be expected to provide an ideal molecular targets for the treatment of glioma.