生长素对5氟尿嘧啶化疗大鼠肠道屏障损伤保护作用

Protective effect of ghrelin on 5-fluorouracil-induced damage on rat gut barrier

目的观察生长素对5氟尿嘧啶化疗大鼠肠道屏障损伤保护作用。方法将40只雄性SpragueDawley大鼠随机分成4组,每组10只。其中对照组(0.9%生理盐水0.5 mL腹腔注射,每12 h注射1次,连续注射6次)、5FU造模组(5-FU腹腔注射1次+0.9%生理盐水0.5 mL腹腔注射,每12 h 1次,连续6次)、ghrelin阳性对照组(生长素腹腔注射,每12 h 1次,连续6次)和生长素治疗组(5-FU腹腔注射1次+生长素腹腔注射,每12 h 1次,连续6次),采用EDU法测定小肠上皮细胞增殖,Tunnel法检测小肠上皮细胞凋亡,并检测大鼠小肠黏膜形态和损伤指标。结果生长素治疗组大鼠的组织病理、血清DAO和D-乳酸都有优于5-FU组,小肠黏膜细胞凋亡低于5-FU组,而其他均高于5FU组,差异均有统计学意义(P<0.01)。结论生长素对5-FU诱导的大鼠小肠黏膜损伤通过减少凋亡增加增生而起保护作用。

Objective To observe the protective effect of auxin on intestinal barrier injury in rats after 5-fluorouracil chemotherapy.MethodsForty male Sprague-Dawley rats were randomly divided into four groups,each group included ten rats. Control group(0.9% saline 0.5 mL intraperitoneal injection q12 h × 6 times),5-FU model group(5-FU intraperitoneal injection once + 0.9% saline 0.5 mL intraperitoneal injection q12 h × 6 times),ghrelin positive control group(auxin intraperitoneal injection q12 h × 6 times),auxin treatment group(5-FU intraperitoneal injection once + auxin intraperitoneal injection q12 h × 6 times).ResultsThe histopathology,serum DAO and D-lactic acid of rats in auxin treatment group were better than those in 5-FU group,and the apoptosis of small intestinal mucosa cells was lower than that in 5-FU group,while the others were higher than those in 5-FU group(P<0.01).ConclusionAuxin has protective effect on small intestinal mucosal injury induced by 5-FU in rats by reducing apoptosis and increasing proliferation.