甲泼尼龙对单侧输尿管梗阻诱导的肾纤维化的影响

Effect of Methylprednisolone on Unilateral Ureteral Obstruction-induced Renal Fibrosis

为探讨甲泼尼龙在小鼠肾纤维化中的作用,本文通过单侧输尿管梗阻(unilateral ureteral obstruction,UUO)实验建立肾纤维化模型。实验将25只雄性Balb/c小鼠随机分为假手术组、模型组、低剂量组、中剂量组、高剂量组。手术后第2天给药,14 d后,使用HE染色和Masson染色检测各组小鼠肾脏病理变化情况。Western blotting和IHC染色检测各组梗阻侧肾脏中Col-1、Col3A1、FN、α-SMA的表达情况以及TGF-β/Smad和NF-κB信号通路中蛋白的表达情况。结果显示UUO会导致小鼠肾间质纤维化,甲泼尼龙能显著减弱UUO小鼠肾间质细胞外基质Col-1、Col3A1、FN、α-SMA的生成,下调TGF-β/Smad和NF-κB信号通路中蛋白的表达。由此可知,甲泼尼龙对小鼠肾纤维化有明显的改善作用,它通过抑制TGF-β/Smad和NF-κB信号通路来减轻UUO诱导的肾纤维化。

To investigate the role of methylprednisolone in renal fibrosis in mice,A renal fibrosis model was established by Unilateral Ureteral Obstruction(UUO) experiment. Twenty-five male Balb/c mice were randomly divided into sham operation group, model group, low dose group, medium dose group and high dose group. The drug was administered on the second day after the surgery, and 14 days later, renal pathological changes were detected by HE staining and Masson staining. Western blotting and IHC staining were used to detect the expression of Col-1, Col3 A1, FN and α-SMA in the obstructed kidneys of each group and to detect the expression of TGF-β/Smad and NF-κB signaling pathways. The results showed that UUO can cause renal interstitial fibrosis. Methylprednisolone significantly attenuated the production of extracellular matrix Col-1, Col3 A1, FN and α-SMA in UUO mice, and down-regulated expression of the protein in TGF-β/Smad and NF-κB signaling pathways. It can be seen that methylprednisolone has a significant improvement in renal fibrosis in mice, which reduces UUO-induced renal fibrosis by inhibiting TGF-β/Smad and NF-κB signaling pathways.