ELISA与ECLIA检验在乙肝标志物诊断中的应用分析

A Comparative Analysis of ELISA and ECLIA in the Diagnosis of Hepatitis B Markers

目的对照分析ELISA与ECLIA检验在乙肝标志物诊断中的应用。方法从本院在2017年1月—2018年6月收治的乙型肝炎患者100例为主要研究对象,使用随机筛选方法将这些研究对象随机将其分为观察组和对照组,每组分配50例患者,观察组患者检验方式为化学发光免疫分析技术(ECLIA),对照组检验方式为酶联免疫吸附试验(ELISA),对两组患者的检验结果进行对照分析。结果通过对两组乙型肝炎患者的血清标志物进行检验对比,发现观察组发现的乙型肝炎患者乙型肝炎表面抗原阳性率、乙型肝炎e抗原阳性率、乙型肝炎e抗体阳性率、抗乙型肝炎核心抗体阳性率、抗表面抗体阳性率分别为84.00%、82.00%、82.00%、80.00%和78.00%高于对照组的62.00%、56.00%、60.00%、60.00%和50.00%,与对照组差异具有统计学意义(P<0.05)。结论通过ELISA与ECLIA检验在乙肝标志物诊断中的应用对照分析表明,在进行乙肝标志物诊断过程中使用ECLIA的检出率高于ELISA。

Objective To compare the application of ELISA and ECLIA test in the diagnosis of hepatitis B markers.Methods 100 patients with hepatitis B admitted to our hospital from January 2017 to June 2018 were selected as the main subjects.They were randomly divided into observation group and control group by random screening.50 patients were assigned,and the test group was chemiluminescence immunoassay(ECLIA).The control group was tested by enzyme-linked immunosorbent assay(ELISA).The test results of the two groups were compared.Results By testing the serum markers of two groups of hepatitis B patients,the positive rate of hepatitis B surface antigen,the positive rate of hepatitis B e antigen,the positive rate of hepatitis B e antibody,and the antibiotics in the observation group were compared.The positive rate of hepatitis B core antibody and the positive rate of anti-surface antibody were 84.00%,82.00%,82.00%,80.00% and 78.00%,respectively,which were higher than 62.00%,56.00%,60.00%,60.00% and 50.00% of the control group.The control group had significant differences(P<0.05).Conclusion The comparative analysis of ELISA and ECLIA test in the diagnosis of hepatitis B markers shows that the detection rate of ECLIA in the diagnosis of hepatitis B markers is higher than ELISA,which is worthy of promotion in clinical practice.