附睾精子和睾丸精子微量冷冻技术流程研究

Establishment of Microcryopreservation Technology for Epididymis Sperm and Testicular Sperm

目的建立附睾精子和睾丸精子微量冷冻技术流程,为今后的临床工作提供有价值的参考依据。方法选择2017年1月—2018年6月间10例经皮附睾精子抽吸术(PESA)或经皮睾丸精子抽吸术(TESA)患者作为研究对象,患者精子分别采取自制冷冻管微量冷冻法和商品化冷冻管快速冷冻法进行冷冻、复苏,而后对两种方法的应用效果进行对比分析,建立附睾精子和睾丸精子微量冷冻技术流程。结果自制冷冻管微量冷冻法组精子复苏存活率、精子活动率较商品化冷冻管快速冷冻法显著升高(P<0.05),但临床妊娠率没有显著性差异(P>0.05)。结论附睾精子和睾丸精子微量冷冻技术可有效提高冷冻精子的复苏存活率、精子活动率,精子用量少,操作方便简洁,有利于男性生育力的保存,值得关注并推广。

Objective To establish the technological process of microcryopreservation of epididymal sperm and testicular sperm,and to provide valuable reference for clinical work in the future.Methods From January 2017 to June 2018,10 patients with percutaneous epididymal sperm aspiration(PESA)or percutaneous testicular sperm aspiration(TESA)were selected as the study subjects.The sperm of the patients were frozen and resuscitated by self-made cryosurgery tube micro-freezing method and commercial cryosurgery tube rapid freezing method respectively.Then the application effects of the two methods were compared and analyzed to establish epididymal sperm and testis.The technological process of sperm microfreezing was constructed.Results The survival rate of sperm resuscitation and sperm motility in self-made cryopreservation tube group were significantly higher than those in commercial cryopreservation tube group(P<0.05),but there was no significant difference in clinical pregnancy rate(P>0.05).Conclusion Microcryopreservation of epididymal sperm and testicular sperm can effectively improve the survival rate of cryopreserved sperm,sperm motility rate,low sperm dosage,simple operation,conducive to the preservation of male fertility,worthy of attention and promotion.