超声-表面活性剂提取青钱柳总黄酮的工艺优化

Optimization of ultrasonic-surfactant extraction of total flavonoids from Cyclocarya paliurus

目的优化表面活性剂结合超声提取青钱柳中的总黄酮的工艺。方法以总黄酮含量为指标,在单因素实验的基础上运用Box-Behnken响应面法(response surface methodology,RSM)设计四因素三水平实验获得最佳提取条件。结果青钱柳总黄酮的最佳提取工艺为:吐温80浓度5%、液料比41:1(V:m)、超声时间26 min和超声温度60℃。此条件下,实际测得总黄酮含量为(76.27±0.27)mg/g,与预测值77.32 mg/g非常接近,是比较理想的模型。结论超声与表面活性剂的结合可以较好地提取青钱柳中的总黄酮。

Objective To optimize extraction technology of total flavonoids in Cyclocarya paliurus by surfactant combined with ultrasonication. Methods Using the total flavonoid content as an indicator,based on the single factor experiment,the Box-Behnken response surface method(RSM) was used to design the four-factor and three-level tests to obtain the best extraction conditions. Results The optimum extraction conditions of total flavonoids from Cyclocarya paliurus were as follows: Tween 80 concentration 5%,liquid to material ratio 41:1(V:m),ultrasonic time 26 min and ultrasonic temperature 60 ℃. Under this condition,the actual content of total flavonoids was(76.27±0.27) mg/g,which was very close to the predicted value of 77.32 mg/g,and indicated that it was an ideal model. Conclusion The combination of ultrasound and surfactant can better extract the total flavonoids from Cyclocarya paliurus.