基质辅助激光解析电离飞行时间质谱法鉴定乳粉中致病菌

Identification of milk powder pathogenic bacteria by matrix assisted laser analytical ionization time-of-flight mass spectrometry

目的采用基质辅助激光解析电离飞行时间质谱法鉴定乳粉中的几种食源性致病微生物,评价此技术在乳粉安全评价中的应用前景。方法以乳粉中常见几种食源性致病菌(阪崎肠杆菌、肺炎克雷伯氏菌、产酸克雷伯氏菌)为目标菌,同时分离未知菌,通过基质辅助激光解吸附电离飞行时间质谱进行鉴定。结果共从80个样品中分离到3种细菌,分别为阪崎肠杆菌、肺炎克雷伯氏菌,阴沟肠杆菌。结论基质辅助激光解吸附电离飞行时间质谱能快速对未知细菌进行鉴定,本次实验从乳粉上分离到的细菌,多数为条件致病菌,存在一定的食用风险。

Objective To identify several foodborne pathogenic microorganisms in milk powder by matrix-assisted laser analytical ionization time-of-flight mass spectrometry and evaluate the application prospect of this technique in milk powder safety evaluation. Methods Several common food-borne pathogens(Enterobacter sakazakii,Klebsiella pneumoniae,Klebsiella pneumoniae) in milk powder were selected as target bacteria,and unknown bacteria were isolated and identified by matrix7 gv-assisted laser desorption ionization time-of-flight mass spectrometry. Results A total of 3 bacteria were isolated from 80 samples,they were Enterobacter sakazakii,Klebsiella pneumoniae,Enterobacter cloacae,respectively. Conclusion Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry can quickly identify unknown bacteria. Most of the bacteria isolated from milk powder in this experiment are conditional pathogens,which have certain food risks.