氨基甲酸酯类农药残留质控样品的制备与评价-以灭多威为例

Preparation and evaluation of quality control samples of carbamate pesticide residues-a case study of methomyl

目的建立一种蔬菜冻干粉中氨基甲酸酯类农药残留质控样品的制备方法并对制备技术进行评价。方法以圆白菜为基质,灭多威为目标物,采用真空冷冻干燥,基质添加的方式制备质控样品。采用液相色谱-串联质谱法对质控样品中目标物含量进行测定。通过随机抽样测定以评估样品的均匀性,通过观察样品12个月内目标物检测结果变化以评估样品的稳定性。结果本方法制备的灭多威质控样品均匀性和稳定性良好。蔬菜冻干粉质控样品便于贮藏、运输且在常温下能够长期稳定保存。样品中灭多威的制备值与测得值较好吻合。结论本研究确立了蔬菜冻干粉中灭多威质控样品的制备方法,所建立的制备技术可以确保质控样品定值结果的准确性,为氨基甲酸酯类农药残留质控样品制备方法的开发提供技术支持。

Objective To establish a preparation method for carbamate pesticide residue quality control samples in vegetable freeze-dried powder,and to evaluate the preparation technology. Methods The quality control samples were prepared by using cabbage as substrate and methomyl as the target. Quality control samples were prepared by vacuum freeze-drying and matrix addition Liquid chromatography-tandem mass spectrometry was used to determine the content of the target substance in the controlled samples. The homogeneity of the samples was evaluated by random sampling,and the stability of the samples was evaluated by observing the changes in the test results of the objects within 12 months of the samples. Results The prepared quality control samples had good homogeneity and stability. It was convenient for the samples to storage and transportation and the concentration level of target in samples,which were stored at room temperature,was stable during the testing time. The measured value of methomyl in prepared quality control samples agreed with the target value of preparation. Conclusion This study established the feasibility of the study on the quality control samples of vegetable freeze-dried powder,and the established preparation technology could ensure the accuracy of the fixed value results of the quality control samples,providing technical support for the development of the quality control samples of carbamate pesticide residues.