不同环境下稻米品质性状QTL的检测及稳定性分析

Identification and Stability Analysis of QTL for Grain Quality Traits Under Multiple Environments in Rice

【目的】挖掘新的稻米品质性状QTL并利用分子育种技术改良稻米品质。【方法】利用构建的一套以籼稻香型恢复系昌恢121为背景亲本,以优质粳稻越光为供体亲本的染色体片段代换系(CSSLs)为材料,在4个环境下对稻米品质性状进行QTL检测及稳定性分析。【结果】在4个环境下共检测到44个QTL,其中6个QTL能在多个环境下被检测到;第2、3、5、6和10染色体上存在多效QTL簇,对稻米品质性状具有明显的调控作用;第1、6和12染色体上7个QTL能在不同环境下稳定表达。【结论】第1染色体RM3143-RM1117区间qPGWC1和第12染色体RM3331-RM5479区间qPaT12是两个新的稳定表达QTL。

【Objective】Our aim is to explore new QTLs controlling rice quality and to use molecular breeding technology to improve rice quality.【Method】Using fragrant restorer line Changhui 121 as the background parent and high-quality japonica rice Koshihikari as the donor parent,a set of chromosome segment substitution lines(CSSLs)was constructed for QTL detection and stability analysis of rice quality traits in four environments.【Result】A total of 44 QTLs were detected in four environments,six of which could be detected in multiple environments.There were multiple QTL clusters on chromosome 2,3,5,6 and 10,which have obvious regulatory effects on rice quality.Seven QTLs on chromosomes 1,6 and 12 can be expressed stably in different environments.【Conclusion】The qPGWC1 of chromosome 1(RM3143-RM1117)and the qPaT12 of chromosome 12(RM3331-RM5479)are two new stable QTL.