3种梅毒血清学检测方法的应用评价

Application Evaluation of Three Syphilis Serological Detection Methods

目的研究3种梅毒血清学检测方法的应用。方法方便选取该院2016年2月—2019年4月收治的梅毒患者80例作为研究对象,以感染梅毒情况为基点随机分感染组与未感染组,各40例,均采用甲苯胺红不加热血清试验、梅毒螺旋体明胶凝集试验与梅毒螺旋体免疫印迹法,比较两组梅毒抗体阳性情况、3种血清学实验方法的检测效能与实验方法的应用价值。结果感染组抗体阳性率80.00%、90.00%与97.50%高于未感染组12.50%、2.50%与0.00%;感染组的免疫印迹法阳性率(97.50%)高于甲苯胺红不加热血清试验(80.00%)与梅毒螺旋体明胶凝集试验(90.00%),差异有统计学意义(χ^2=6.383,P=0.041);甲苯胺红不加热血清试验敏感性为80.00%、特异性为90.00%、阳性预测值为88.88%、阴性预测值为81.81%;梅毒螺旋体明胶凝集试验敏感性为87.50%、特异性为97.50%、阳性预测值为97.22%、阴性预测值为88.63%;免疫印迹法敏感性为92.50%、特异性为100.00%、阳性预测值为100.00%、阴性预测值为93.02%,与甲苯胺红不加热血清试验与梅毒螺旋体明胶凝集试验比较,免疫印迹法的敏感性、特异性、阳性预测值与阴性预测值均高,差异有统计学意义(P<0.05)。结论对梅毒患者采用甲苯胺红不加热血清试验、梅毒螺旋体明胶凝集试验与免疫印迹法检测能早期确诊病情,避免发生误诊或漏诊,为后续治疗提供参考,值得借鉴。

Objective To study the application of three serological tests for syphilis.Methods Eighty patients with syphilis admitted to the hospital from February 2016 to April 2019 were convenient enrolled in the study.The infection was treated with syphilis as the basis for random infection and 40 cases of uninfected group.The test,syphilis spirogel gelatin agglutination test and Treponema pallidum immunoblotting,comparing the serotype antibody positive status of the two groups,the detection efficiency of the three serological test methods and the application value of the experimental method.Results The positive rate of antibody in the infected group 80.00%,90.00%and 97.50% was higher than 12.50%,2.50%and 0.00%in the uninfected group;the positive rate of immunoblotting in the infected group(97.50%)was higher than that in the toluidine red non-heated serum test(80.00%)and syphilis spirogel gelatin agglutination test(90.00%),,the difference was statistically significant(χ^2=6.383,P=0.041)toluidine red unheated serum test sensitivity is 80.00%,specificity is 90.00%,positive predictive value is 88.88%,negative The predicted value was 81.81%;the sensitivity of the syphilis spirogel gelatin test was 87.50%,the specificity was 97.50%,the positive predictive value was 97.22%,the negative predictive value was 88.63%;the immunoblotting sensitivity was 92.50%,and the specificity was 100.00.%,positive predictive value is 100.00%,negative predictive value is 93.02%,compared with toluidine red unheated serum test and treponema pallidum gelatin agglutination test,the sensitivity,specificity,positive predictive value and negative predictive value of immunoblotting are both High,the difference was statistically significant(P<0.05).Conclusion Toluidine red unheated serum test,syphilis spirogel gelatin agglutination test and immunoblotting test for syphilis patients can diagnose the disease early and avoid it.Misdiagnosis or missed diagnosis provides reference for follow-up treatment and is worth learning.