Dicer mRNA在肥胖高血压内脏白色脂肪组织中的表达

Dicer expression in visceral white adipose tissue of obese hypertension patients

目的研究肥胖高血压患者内脏白色脂肪组织Dicer的表达水平。方法收集314例行腹部择期手术患者内脏白色脂肪组织,检测其中Dicer mRNA的表达水平,分析年龄、肥胖、单纯高血压、肥胖高血压与内脏白色脂肪组织Dicer mRNA表达的关系。结果与31~40岁患者相比,41~50岁,51~60岁,60岁以上患者内脏白色脂肪组织Dicer mRNA表达增加,差异有统计学意义(P<0.05);与41~50岁患者相比,60岁以上老年人内脏白色脂肪组织中Dicer mRNA表达增加,差异具有统计学意义(P<0.05);与51~60岁患者相比,60岁以上老年人内脏白色脂肪组织中Dicer mRNA表达增加,差异具有统计学意义(P<0.05)。60岁以上患者中,与正常血压患者相比,高血压患者内脏白色脂肪组织中Dicer mRNA表达下降,差异有统计学意义(P<0.05)。60岁以下患者中,与正常体重正常血压者相比,单纯高血压和肥胖高血压患者内脏白色脂肪组织中Dicer mRNA表达降低,差异有统计学意义(P<0.05);且与单纯高血压患者相比,肥胖高血压患者内脏白色脂肪组织中Dicer mRNA表达降低,差异具有统计学意义(P<0.05)。结论内脏白色脂肪组织Dicer mRNA表达降低与高血压,尤其是与肥胖高血压密切相关。

Objective To investigate the expression of Dicer in visceral white adipose tissue of hypertension patients.Methods The visceral white adipose tissues from 314 patients who underwent elective abdominal surgery in our hospital were collected to detect the expression of Dicer by quantitative real-time PCR(qRT-PCR).And their clinical pathological data including age,height,weight and arterial blood pressure were collected for correlation analysis.Results Compared with the patients between 31 and 40 years old,the expression level of Dicer mRNA in visceral white adipose tissue of patients between 41 and 50 years old,between 51 and 60 years old,more than 60 years old increased,the difference was statistically significant(P<0.05);compared with the patients between 41 and 50 years old,the expression level of Dicer mRNA in visceral white adipose tissue of patients more than 60 years old increased,the difference was statistically significant(P<0.05);compared with the patients between 51 and 60 years old,the expression level of Dicer mRNA in visceral white adipose tissue of patients more than 60 years old increased,the difference was statistically significant(P<0.05).In the patients more than 60 years old,compared with the patients of normal blood pressure,the expression level of Dicer mRNA in visceral white adipose tissue of patients with hypertensive decreased,the difference was statistically significant(P<0.05).In the patients less than 60 years old,compared with the patients of normal blood pressure and weight,the expression level of Dicer mRNA in visceral white adipose tissue of patients with simple hypertension and obesity hypertension decreased,the difference was statistically significant(P<0.05);compared with the patients with simple hypertension,the expression level of Dicer mRNA in visceral white adipose tissue of patients with obesity hypertensive decreased,the difference was statistically significant(P<0.05).Conclusion The decreased expression of Dicer mRNA in the visceral white fat tissue was involved in occurrence and development of hypertension,especially obese hypertension.