原发免疫性血小板减少症患者ANA及ANAs筛查结果分析

Analysis on results of ANA and ANAs screening for patients with primary immune thrombocytopenia

目的 探讨间接免疫荧光法检测的抗核抗体(ANA)及免疫印迹法检测的抗核抗体谱(ANAs)在原发免疫性血小板减少症(ITP)患者中的特点。方法 对123例初诊ITP的患者采用间接免疫荧光法检测ANA,免疫印迹法检测ANAs,并对结果进行回顾性分析。结果 123例ITP患者中ANAs阳性率高于ANA,差异有统计学意义(P<0.05);ANAs 15种抗体中抗干燥综合征抗原A抗体(SSA)的阳性率最高,其次为Ro52,差异有统计学意义(P<0.05),抗Scl-70、PM-Sc及Jo-1均为阴性,其余10种抗体阳性率不一,但是相互之间对比差异无统计学意义(P>0.05);抗体联合表达阳性率高于单项表达,差异有统计学意义(P<0.01)。结论在ITP患者ANA及ANAs阳性率不同,ANAs表达主要以单项及联合抗体表达为主,但是联合表达高于单项表达。

Objective To explore expression characteristics of antinuclear antibody(ANA)by indirect immunofluorescence and antinuclear antibody spectrum(ANAs)by Western blot in patients with newly diagnosed primary immune thrombocytopenia(ITP).Methods A total of 123 patiens of newly diagnosed ITP were enrolled in this study.ANA and ANAs examination in all patients had been carried out by indirect immunofluorescence and by Western blot respectively.Characteristic expression of ANA and ANAs in the patients was retrospectively analyzed.Results The ANAs positive rate ANAs in 123 ITP patients was higher than that of ANA,and the difference was statistically significant(P<0.05).The anti SSA of 15 ANA antibodies was the highest,followed by Ro52,and the difference was statistically significant(P<0.05).There was no expression of anti-Scl-70,PM-Sc and Jo-1.The positive rates of the other 10 antibodies were different,but there was no significant difference between them(P>0.05).The positive rate of antibody combined expression was higher than that of single expression,and the difference was statistically significant(P<0.01).Conclusion The positive rates of ANA and ANAs were different in ITP patients.The expression of ANAs was mainly expressed by single antibody and combined antibody,but the expression of combined antibody was higher than that of single antibody.