人附睾蛋白4通过上调MMP9及VEGF降低顺铂对卵巢癌细胞增殖和迁移的影响

HE4 decreases the effect of cisplatin on proliferation and migration of ovarian cancer cell by up-regulating MMP9 and VEGF

目的 探讨人附睾蛋白4(HE4)对顺铂作用后卵巢癌细胞增殖和迁移的影响及潜在机制。方法 构建HE4质粒,脂质体Lipofectamine 2000转染人卵巢癌细胞系HO8910PM,通过Western blot验证稳定转染细胞株构建成功。通过CCK8实验检测HE4过表达对顺铂作用后细胞增殖的影响;通过Transwell实验检测HE4过表达对顺铂作用后细胞迁移的影响;同时通过Western blot实验检测促迁移相关蛋白基质金属蛋白酶9(MMP9)和血管内皮生长因子(VEGF)的表达。结果 过表达HE4人卵巢癌细胞系HO8910PM构建成功;相比于转染空载体的HO8910PM细胞,HE4可明显降低顺铂对细胞增殖的抑制作用,促进HO8910细胞的增殖;同时,HE4过表达细胞中MMP9及VEGF蛋白表达水平也相应提高。结论 HE4可能通过上调MMP9及VEGF表达降低顺铂对卵巢癌细胞增殖和迁移的影响。

Objective To investigate the effect and mechanism of Human epididymal protein 4(HE4)on proliferation and migration of ovarian cancer cell after cisplatin treatment.Methods HE4 plasmid was constructed and Lipofectamine 2000 was transfected into human ovarian cancer cell line HO8910PM.Western blot was used to verify the successful construction of stable cell line.The effect of HE4 overexpression on cisplatin induced cell proliferation was detected by CCK8 assay,the effect of HE4 overexpression on cell migration after cisplatin treatment was detected by Transwell test,and the expression of matrix metalloproteinase-9 and vascular endothelial growth factor(VEGF)were detected by Western blot.Results Compared with HO8910PM cells transfected with empty vector,HE4 could significantly reduce the inhibition of cisplatin on cells and promote the proliferation of HO8910 cells.At the same time,the expression of MMP9 and VEGF protein in the overexpressed cells of HE4 were also increased.Conclusion HE4 decreases the effect of cisplatin on proliferation and migration of ovarian cancer cell by up-regulating MMP9 and VEGF.