循环microRNA-95在前列腺癌早期精准诊断和预后判断中的作用

Role of circulating microRNA-95 in early accurate diagnosis and prognosis of prostate cancer

目的:探讨循环microRNA(以下简称miR-95)在前列腺癌早期精准诊断和预后判断中的作用。方法:通过qPCR检测60例前列腺癌患者、60例前列腺增生患者及60例健康男性血清miR-95的相对表达水平,分析血清miR-95的相对表达水平与临床病理特征的关系,并构建受试者工作特征(receiver operating characters,ROC)曲线,分析血清miR-95对于前列腺癌诊断的敏感度和特异度。结果:前列腺癌患者血清miR-95的表达水平较前列腺增生及健康男性增高,差异有统计学意义(P<0.05),而前列腺增生患者血清miR-95的表达水平和健康男性中的表达无明显统计学差异(P>0.05)。血清miR-95表达水平与前列腺癌临床病理分期、Gleason评分及骨转移显著相关(P<0.05);与年龄、前列腺特异性抗原水平无关(P>0.05)。ROC曲线显示miR-95能够较好的区分前列腺癌与前列腺增生及健康男性,曲线下面积分别为0.826(95%CI:0.792~0.918)和0.808(95%CI:0.745~0.919)。结论:miR-95在前列腺癌患者血清中高表达,与前列腺癌进展有关,有望作前列腺癌早期精准诊断和预后的标志物。

Objective:To investigate the role of circulating microRNA-95 in early accurate diagnosis and prognosis of prostate cancer.Methods:qPCR was used to detect the relative expression level of serum microRNA-95 in 60 patients with prostate cancer,60 patients with benign prostatic hyperplasia and 60 healthy men.The relationship between the relative expression level of serum microRNA-95 and clinicopathological characteristics was analyzed.The ROC curve was constructed to analyze the sensitivity and specificity of serum microRNA-95 in the diagnosis of prostate cancer.Results:The expression level of serum microRNA-95 in prostate cancer patients was higher than that in benign prostatic hyperplasia and healthy men(P<0.05),but there was no significant difference in the expression level of serum microRNA-95 between benign prostatic hyperplasia patients and healthy men(P>0.05).Serum level of microRNA-95 was significantly correlated with clinical pathological stage,Gleason score and bone metastasis of prostate cancer(P<0.05),but not with age and prostate specific antigen(P>0.05).ROC curve showed that microRNA-95 could differentiate prostate cancer from benign prostatic hyperplasia and healthy males.The area under the curve was 0.826(95%CI:0.792–0.918)and 0.808(95%CI:0.745–0.919),respectively.Conclusion:The high expression of microRNA-95 in the serum of prostate cancer patients is related to the progression of prostate cancer,and it is expected to be a marker of early accurate diagnosis and prognosis of prostate cancer.