血清FFA、β-arrestin2、RBP4在2型糖尿病患者中的表达及临床意义

Expression and clinical significance of serum FFA, β-arrestin2 and RBP4 in patients with type 2 diabetes mellitus

目的探讨血清游离脂肪酸(FFA)、β抑制蛋白2(β-arrestin2)、视黄醇结合蛋白4(RBP4)在2型糖尿病(T2DM)患者中表达及临床意义。方法选取2016年2月~2018年12月苏州大学附属张家港医院(以下简称“我院”)收治的123例T2DM患者作为观察组,将观察组根据体重指数(BMI)结果分为T2DM肥胖组(BMI≥28 kg/m 2,n=62)和T2DM非肥胖组(BMI<28 kg/m 2,n=61),另选取同期来我院行健康体检的志愿者60名作为对照组。比较三组研究对象血清FFA、β-arrestin2、RBP4水平、糖脂代谢水平;采用Pearson相关性分析血清FFA、β-ar restin2、RBP4水平与糖脂代谢水平的相关性。结果T2DM肥胖组、T2DM非肥胖组血清FFA、RBP4水平高于对照组,且T2DM肥胖组高于T2DM非肥胖组(P<0.05);T2DM肥胖组、T2DM非肥胖组血清β-arrestin2水平低于对照组,且T2DM肥胖组低于T2DM非肥胖组(P<0.05)。T2DM非肥胖组、T2DM肥胖组糖化血红蛋白(HbA 1c)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、三酰甘油(TG)、胰岛素抵抗指数(HOMA-IR)、BMI均高于对照组,且T2DM肥胖组高于T2DM非肥胖组(P<0.05);而三组高密度脂蛋白胆固醇(HDL-C)比较差异无统计学意义(P>0.05)。T2DM患者血清FFA和RBP4与HbA 1c、TC、TG、LDL-C、HOMA-IR、BMI均呈正相关(r>0,P<0.05),β-arrestin2与HbA 1c、TC、TG、LDL-C、HOMA-IR、BMI均呈负相关(r<0,P<0.05);FFA、RBP4和β-ar-restin2与HDL-C均无相关性(P>0.05)。结论T2DM患者中血清FFA、β-arrestin2、RBP4水平均存在异常表达,三者可能共同促进T2DM的病情进展。

Objective To investigate the expression and clinical significance of serum free fatty acids(FFA),βinhibi-tion of protein 2(β-arrestin2),retinol-binding protein 4(RBP4)in patients with type 2 diabetes mellitus(T2DM).Methods From February 2016 to December 2018,123 T2DM patients admitted to Zhangjiagang Hospital Affiliated To Soochow University(hereinafter referred to as“our hospital”)were selected as the observation group.The observation group was divided into the obese T2DM group(BMI≥28 kg/m 2,n=62)and the non-obese T2DM group(BMI<28 kg/m 2,n=61)according to body mass index(BMI).In addition,60 volunteers who came to our hospital for physical examina-tion during the same period were selected as the control group.The serum levels of FFA,β-arrestin2,RBP4 and glu-cose and lipid metabolism of the three groups were compared.Pearson correlation was used to analyze the correlation between the serum levels of FFA,β-arrestin2 and RBP4 and the level of glucose and lipid metabolism.Results The serum levels of FFA and RBP4 in the T2DM obesity group and the T2DM non-obesity group were higher than those in the control group,and those in the T2DM obesity group were higher than those in the T2DM non-obesity group(P<0.05).The level ofβ-arrestin2 in the T2DM obesity group and the T2DM non-obesity group was lower than that in the control group,and that in the T2DM obesity group was lower than that in the T2DM non-obesity group(P<0.05).The glycosylated hemoglobin(HbA 1c),total cholesterol(TC),low density lipoprotein cholesterin(LDL-C),triglyceride(TG),insulin resistance index(HOMA-IR)and BMI in the T2DM obesity group and the T2DM non-obesity group were higher than the control group,and those in the T2DM obesity group were higher than those in the T2DM non-obesity group(P<0.05).However,there was no statistically significant difference in high-density lipoprotein cholesterol(HDL-C)between the three groups(P>0.05).Serum FFA and RBP4 of T2DM patients was pos-itively correlated with HbA 1c,TC,TG,LDL-C,HOMA-IR and BMI(r>0,P<0.05),β-arrestin2 was negatively cor-related with HbA 1c,TC,TG,LDL-C,HOMA-IR and BMI(r<0,P<0.05).FFA,RBP4 andβ-arrestin2 were not cor-related with HDL-C(P>0.05).Conclusion In T2DM patients,the serum levels of FFA,β-arrestin2 and RBP4 were all abnormally expressed,which may jointly promote the disease progression of T2DM.