芪参益气滴丸治疗射血分数保留心力衰竭的网络药理学研究

Network pharmacological research of Qishen Yiqi Dropping Pills in the treatment of heart failure with preserved ejection fraction

目的基于网络药理学方法探讨芪参益气滴丸治疗射血分数保留心力衰竭(HFpEF)的潜在活性成分和作用机制。方法采用BATMAN-TCM平台收集黄芪、丹参、三七、降香的活性成分和作用靶点信息,GeneCards和PALM-IST平台收集HFpEF疾病靶点基因,搜索日期为2019年3月4日;采用Cytoscape 3.2.1构建"药物-活性成分-靶点"网络并利用network analyzer插件进行拓扑结构分析,采用STRING数据库进行基因互作分析,采用Metascape数据库进行基因本体论(GO)分析及KEGG信号通路富集分析。结果共获得芪参益气滴丸治疗HFpEF的潜在活性成分95个,潜在靶点基因262个;基因富集分析显示芪参益气滴丸主要通过调控炎性反应、氧化应激、心肌纤维化、细胞凋亡等生物学过程发挥治疗HFpEF的作用。结论本研究从网络药理学角度初步阐释了芪参益气滴丸治疗HFpEF的潜在活性成分和作用机制,揭示了其多成分、多靶点、多途径整合调节的效应特点,为后续开展基础实验研究提供了线索和依据。

Objective To explore the potential active components and mechanisms of Qishen Yiqi Dropping Pills on the treatment of heart failure with preserved ejection fraction(HFpEF) through network pharmacology-based techniques.Methods The active components and target information of Astragalus propinquus Schischkin, Salvia miltiorrhiza Bunge,Ranax Notoginseng(Burk.)F. H. Chen, and Dalbergiae Odoriferae T. Chen were collected by BATMAN-TCM platform.Genecards database and PALM-IST platform was used to collect target genes related to HFpEF. Search date was March4, 2019. Drug-active components-target network was constructed and visualized by Cytoscape 3.2.1. Topological characteristic of the nodes in the network was analyzed using network analyzer. Target genes interaction was analyzed by STRING database while gene ontology(GO) and pathways enrichment were analyzed by Metascape database.Results A total of 95 potential active components and 262 potential target genes of HFpEF were obtained from Qishen Yiqi Dropping Pills. Gene enrichment analysis showed that Qishen Yiqi Dropping Pills played a role in treatment of HFpEF by regulating biological process including inflammatory response, oxidative stress, myocardial fibrosis and apoptosis. Conclusion This study preliminarily discussed potential active components and mechanism of Qishen Yiqi Dropping Pills on the treatment of HFpEF on a network pharmacological basis, revealing the multi-component,multi-target and multi-channel integrated regulating effect, and provids theoretical information for further experimental research.