丁香苦苷固体脂质纳米粒在小鼠体内的组织分布

Tissue distribution of syringopicroside solid lipid nanoparticles in mice in vivo

目的研究丁香苦苷固体脂质纳米粒在小鼠体内的组织分布。方法乳化蒸发法制备丁香苦苷固体脂质纳米粒后,小鼠尾静脉注射给予其冻干粉溶液(15 mg/kg)。然后,HPLC法测定不同时间点(10、30、50、70、90 min)血浆、心、肝、脾、肺、肾中丁香苦苷含有量。结果丁香苦苷固体脂质纳米粒在肝脏中的靶向效率大于1,相对摄取率为1.65,靶向增强倍数为1.84,明显高于血浆和其他组织中。结论丁香苦苷主要富集于肝组织,其固体脂质纳米粒可有效提高其肝靶向性和分布。

AIM To study the tissue distribution of syringopicroside solid lipid nanoparticles in mice in vivo.METHODS For the solid lipid nanoparticles prepared by emulsion evaporation method, the freeze-dried powder solution(15 mg/kg) was given to mice by tail intravenous injection. Subsequently, HPLC was adopted in the content determination of syringopicroside in plasma, heart, liver, spleen, lung and kidney at different time points(10, 30, 50, 70, 90 min).RESULTS The targeting efficiency of solid lipid nanoparticles in liver was more than 1, along with the relative intake efficiency and targeted enhancement multiple of 1.65 and 1.84, respectively, which were obviously higher than those in plasma and the other tissues.CONCLUSION Syringopicroside is mainly enriched in liver tissue, whose liver-targeting property and distribution can be effectively enhanced by solid lipid nanoparticles.