苦参碱固体脂质纳米粒的制备和处方优化

Preparation and formulation optimization of matrine solid lipid nanoparticles

目的制备苦参碱固体脂质纳米粒,并进行处方优化。方法选择硬脂酸作为固体脂质,吐温-80作为乳化剂,高压均质法制备苦参碱固体脂质纳米粒。以吐温-80用量、均质压力、药脂比为影响因素,包封率为评价指标,正交试验优化制备工艺。结果最佳处方为吐温-80用量0.3 g,均质压力90 MPa,药脂比1∶5,包封率(94.15±1.44)%。结论该方法简便可靠,可用于制备苦参碱固体脂质纳米粒。

AIM To prepare matrine solid lipid nanoparticles and to make formulation optimization.METHODS Taking stearic acid as solid lipid and Tween-80 as emulsifier, the solid lipid nanoparticles were prepared by high-pressure homogenization method. With Tween-80 consumption, homogenization pressure and drug-lipid ratio as influencing factors, encapsulation efficiency as an evaluation index, the preparation process was optimized by orthogonal test.RESULTS The optimal formulation was determined to be 0.3 g for Tween-80 consumption, 90 MPa for homogenization pressure, and 1∶5 for drug-lipid ratio, the encapsulation efficiency was(94.15±1.44)%.CONCLUSION This simple and reliable method can be used for the preparation of matrine solid lipid nanoparticles.