UPLC-PDA法同时测定复方五凤草液中7种成分

Simultaneous determination of seven constituents in Compound Wufengcao Liquid by UPLC-PDA

目的建立UPLC-PDA法同时测定复方五凤草液(五凤草、白芨、猫爪草)中没食子酸、原儿茶酸、对羟基苯甲酸、咖啡酸、金丝桃苷、槲皮素、柚皮素的含有量。方法该药物80%甲醇提取液的分析采用Waters BEH C18色谱柱(2.1 mm×100 mm, 1.7μm);流动相乙腈-0.1%甲酸,梯度洗脱;体积流量0.3 mL/min;柱温40℃;检测波长266 nm。结果 7种成分在各自范围内线性关系良好(r≥0.999 5),平均加样回收率96.96%~102.26%,RSD 0.65%~2.10%。结论该方法稳定准确,重复性好,可用于复方五凤草液的质量控制。

AIM To establish a UPLC-PDA method for the simultaneous content determination of gallic acid, protocatechuic acid, p-hydroxybenzoic acid, caffeic acid, hyperoside, quercetin and naringenin in Compound Wufengcao Liquid [Euphorbia helioscopia L., Bletilla striata(Thunb.) Reichb.f., Ranunculus ternatus Thunb.].METHODS The analysis of 80% methanol extract of this drug was performed on a 40 ℃ thermostatic Waters BEH C18 column(2.1 mm×100 mm, 1.7 μm), with the mobile phase comprising of acetonitrile-0.1%formic acid flowing at 0.3 mL/min in a gradient elution manner, and the detection wavelength was set at 266 nm.RESULTS Seven constituents showed good linear relationships within their own ranges(r≥0.999 5), whose average recoveries were 96.96%-102.26%with the RSDs of 0.65%-2.10%.CONCLUSION This stable, accurate and reproducible method can be used for the quality control of Compound Wufengcao Liquid.