复方竹节参颗粒对缩宫素诱导的原发性痛经的影响

Effects of Fufang Zhujieshen Granules on oxytocin-induced primary dysmenorrhea

目的探讨复方竹节参颗粒对缩宫素诱导的原发性痛经的影响。方法将KM小鼠、SD大鼠分别随机分为空白组、模型组、痛经宝阳性对照组(小鼠4.11 g/kg、大鼠1.87 g/kg)、复方竹节参颗粒组[低剂量组(小鼠6.17 g/kg、大鼠2.80 g/kg),高剂量组(小鼠18.5 g/kg、大鼠8.41 g/kg)],采用己烯雌酚(小鼠10~20 mg/kg,大鼠5~10 mg/kg)和缩宫素(小鼠20 U/kg,大鼠10 U/kg)诱导原发痛经动物模型,观察记录各组鼠的痛经表观指标(行为反应、饮食、精神状态、毛色、被毛光泽度)并进行评分,采用扭体法观察各组鼠的扭体潜伏期、扭体次数,检测给组鼠血清生化指标(PGE2、NO、MDA)水平。取己烯雌酚诱导后的大鼠子宫,离体加入缩宫素(5 U/L),15 min后加入复方竹节参颗粒(90、270 mg/L),采用PowerLab生物信号记录系统记录体外子宫平滑肌收缩张力曲线,计算收缩张力抑制率。结果与模型组比较,复方竹节参颗粒低剂量(常规剂量)组能延长扭体潜伏期,减少扭体次数(P<0.01),升高PGE2、NO水平(P<0.01),降低MDA水平(P<0.01),提高子宫平滑肌收缩张力抑制率(P<0.01)。结论复方竹节参颗粒具有较好的抗痛经作用,并且在常规剂量使用时疗效更好,可为客观评价其药效作用提供了科学依据。

AIM To investigate the effects of Fufang Zhujieshen Granules(FZGs) on oxytocin-induced primary dysmenorrhea.METHODS KM mice and SD rats were randomly divided into blank group, model group, Tongjingbao dysmenorrhea granules positive control group(4.11 g/kg in mice, 1.87 g/kg in rats), low-dose FZGs group(6.17 g/kg in mice, 2.80 g/kg in rats) and high-dose FZGs group(18.5 g/kg in mice, 8.41 g/kg in rats). And the primary dysmenorrhea animal model was replicated by use of diethylstilbestrol(10~20 mg/kg in mice, 5~10 mg/kg in rats) and oxytocin(20 U/kg in mice, 10 U/kg in rats). Then they were subjected to the following detections: presented multiple symptoms indicators of dysmenorrhea including behavioral response, diet, mental state, coat color, coat gloss, the writhing latent period and writhing times of the primary dysmenorrhea model by writhing method, and serum biochemical indicators(PGE2, NO, MDA) levels. Rats injected with diethylstilbestrol had their uteruses isolated for oxytocin treatment, and 15 min later FZGs(90, 270 mg/L), and the contractile tension curve of isolated uterus were recorded by PowerLab biological signaling system.RESULTS Compared with the model group, the low dose FZGs group extended latent period and decreased writhing times(P<0.01), increased the levels of PGE2 and NO(P<0.01), decreased the levels of MDA in uterine homogenate of animal model(P<0.01) and contraction tension of uterine smooth muscles(P<0.01). CONCLUSION The resultant significant analgesic effect on primary dysmenorrhea models, and a better curative effect at routine dosage. suggest there is scientific evidence for the efficacy evaluation and development of FZGs.