二烯丙基二硫醚对单增李斯特菌毒力基因表达的影响

The Effect of Diallyl Disulfide on the Expression of Virulence Gene in Listeria monocytogenes

二烯丙基二硫醚(DADS)是一种典型的硫醚类香料。该类香料在抗菌消炎,提高机体免疫力,预防和治疗心血管疾病等保健及药理作用方面具有显著效果。而对其在食源性致病菌中的抑制作用及相关机理研究较少。四大食源性致病菌之一单增李斯特菌(Listeria monocytogenes,LM)容易感染食物而引发脑膜炎、败血症及孕妇流产等一系列恶性疾病。本研究以如何高效抑制单增李斯特的生长为出发点,以DADS为研究对象,采用微量肉汤稀释法初步筛选出其对单增能李斯特菌的最小抑菌浓度(MIC),利用RT-qPCR技术考察其对单增李斯特菌毒力基因inlA、hlyA、prfA表达的影响。结果表明:亚抑菌浓度下DADS均可显著抑制毒力基因inlA、hlyA、prfA的表达,且抑制作用呈现一定的浓度依赖性,hlyA及prfA对其作用更敏感。

Diallyl disulfide(DADS)is one typical sulfur flavor.This kind of sulfur flavor showed remarkable effect in antibacterial and anti-inflammatory,improving body immunity,preventing cardiovascular diseases and other health and pharmacological effects.However,most previous studies have focused on the antimicrobial activities of DADS,while relatively limited literature is available on their effect on gene expression.One of the four major foodborne pathogen,Listeria monocytogenes(LM)susceptible to food and lead to meningitis,septicemia and miscarriage and a series of malignant diseases.This study was undertaken to investigate the minimum inhibitory concentration(MIC)of DADS in repressing L.monocytogenes growth in vitro.Additionally,a comprehensive understanding of the transcriptional response of the virulence genes caused by DADS.The results showed that DADS could significantly inhibit the expression of virulence genes inlA,hlyA and prfA under sub-MIC,and the inhibitory effect showed a concentration dependent manner,hlyA and prfA are more sensitive to it.