食源性金黄色葡萄球菌肠毒素基因及其表达检测

The Detection of Enterotoxin Gene and Its Expression of Foodborne Staphylococcus aureus

金黄色葡萄球菌作为一种常见人类病原菌,可通过各种途径污染食品,引发食物中毒。其中,金黄色葡萄球菌肠毒素(SE)的分泌会大大增加其侵袭性和致病力。通过对比肠毒素基因的携带及其表达情况,有助于进一步分析肠毒素表达的环境条件,为产肠毒素金黄色葡萄球菌的防范和控制提供理论和数据支持。本研究采用PCR和3M^TMTecra^TM微孔板法分别检测33株食源性金黄色葡萄球菌中5种传统肠毒素SEA^SEE基因的携带及表达情况,结果显示,该批金黄色葡萄球菌中SE基因的检出率为100%,携带率最高和最低的分别为seb(48.48%,16/33)和see(9.09%,3/33)。而其中SE蛋白得到表达的菌株仅为63.64%(21/33),这表明不是有SE基因携带就一定可以有相应毒素蛋白的表达,可能还需相应的系统调控和适宜的环境因素。

As a common human pathogen,Staphylococcus aureus(S.aureus)can contaminate food through various means and lead to S.aureus food poisoning(SFP).The secretion of Staphylococcal enterotoxins(SE)in S.aureus can greatly increase its invasion and pathogenicity.By comparing the carrying and expression of the SE genes,it’s aimed to lay a foundation for further analysis of the relationship between the expression of SE genes and environmental conditions,so as to provide theoretical and data support for SFP prevention and control.In this study,PCR and 3MTMTecraTM Staph enterotoxin ID test were used to detect the carrying and expression of 5 traditional SE(SEA-SEE)genes in 33 foodborne S.aureus isolates.The results showed that the detection rate of SEs genes was 100%,with the highest and lowest carrying rates of seb(48.48%,16/33)and see(9.09%,3/33),respectively.Only 63.64%(21/33)of these isolates had expressed SEs.It is proved that the carrying of SEs doesn’t mean its correct expression of the toxin protein;corresponding system regulation and appropriate environmental factors may be also needed.