果蔬发酵汁对肠道益生菌及大肠杆菌黏附能力的影响

Effect of fermented juices of fruits and vegetables on the adhesion of intestinal probiotics and Escherichia coli

目的:研究果蔬发酵汁及其提取物对3株肠道益生菌和大肠杆菌黏附能力的影响。方法:采用羧基荧光素琥珀酰亚胺酯(c FDA-SE)对鼠李糖乳杆菌(Lactobacillus rhamnosus)、唾液乳杆菌(Lactobacillus salivarius)、副干酪乳杆菌(Lactobacillus paracasei)和大肠杆菌(Escherichia coli)进行荧光染色,通过流式细胞仪检测荧光值变化,评价果蔬发酵汁及其提取物对肠道菌黏附Caco-2细胞的影响,并检测肠道益生菌和大肠杆菌黏附引起细胞乳酸脱氢酶(LDH)的泄漏量。结果:果蔬发酵汁能明显抑制大肠杆菌的黏附,各提取组分均减少大肠杆菌黏附引起的LDH泄漏(P<0.01),且水溶组分能有效促进肠道益生菌的黏附。结论:果蔬发酵汁具有促进肠道益生菌黏附和抑制肠道非益生菌黏附的作用。

Objective: To study the effect of fermented juice of fruits and vegetables and its extracts on the adhesion of three intestinal probiotics and Escherichia coli. Methods: Lactobacillus rhamnosus, Lactobacillus salivarius, Lactobacillus paracasei and Escherichia coli were treated with carboxyfluoresceinsuccinimidyl ester(cFDA-SE) and the changes of fluorescence values were detected by flow cytometry to evaluatethe effect of the fermented fruit and vegetable juice and its extracts on the adhesion of intestinal bacteria to Caco-2 cells. Meanwhile, the leakage of lactate dehydrogenase(LDH) of cells induced by the adhesion of three intestinal probiotics and Escherichia coli were determined. Results: The fermented juice of fruits and vegetables could inhibit the adhesion of Escherichia coli. The LDH leakage caused by Escherichia coli adhesion was reduced by each extract, and the water soluble extract had better effect on promoting the adhesion of three intestinal probiotics. Conclusion: The fermented juice of fruits and vegetables has the function of promoting the adhesion of intestinal probiotics and inhibiting the adhesion of intestinal non-probiotic bacteria.