驼乳源益生菌对db/db鼠肠道菌群的调节作用

Effects of composite probiotics isolated from fermented camel milk on intestinal microbiota in db/db diabetic mice

目的探究驼乳来源复合益生菌对db/db鼠血糖及肠道菌群的调节作用。方法将60只db/db鼠随机分为5组・每组12只,分别为模型组、二甲双脈组、利拉鲁肽组、低剂量复合益生菌组和高剂量复合益生菌组,12只C57BL/KS小鼠为正常对照组。阳性药组分别灌胃0.3 mg/g二甲双脈及皮下注射0.2 Mg/g利拉鲁肽,低剂量复合益生菌组灌胃乳酸菌1.0X108 CFU/d和酵母菌1.0X106 CFU/d.高剂量复合益生菌组灌胃乳酸菌1.0X1010 CFU/d和酵母菌1.0X108 CFU/d.其他组灌胃生理盐水。血糖仪检测空腹血糖(FBG)及不同时间段血糖水平。ELISA法检测糖化血红蛋白(HbAlc)含量。实时荧光定量PCR检测粪便菌群含量。结果与模型组相比.低、高剂量复合益生菌组FBG水平明显减少.改善葡萄糖耐受(OGTT)能力.降低HbAlc含量。与模型组相比,低、高剂量复合益生菌组显著降低厚壁菌门、放线菌门和大肠埃希菌属水平,增加拟杆菌门、乳杆菌属和双歧杆菌属水平,但对总菌与梭杆菌属无影响。结论驼乳来源复合益生菌可通过调节肠道菌群降低db/db鼠血糖含量。

Objective To explore the effect of composite probiotics isolated from fermented camel milk on the blood glucose and intestinal microbiota in db/db mice. Methods The 60 db/db mice were randomized equally to diabetes model group, metformin group, liraglutide group, low-dose composite probiotics group or high-dose composite probiotics group(12 db/db mice per group). The 12 C57 BL/Ks mice were used as normal control group. Saline was gastrically administered to normal control group and model group. The positive drug groups were respectively given 0.3 mg/g metformin gastrically or 0.2 μg/g liraglutide subcutaneously. The low-dose and high-dose composite probiotics groups were orally given Lactobacillus(1.0×108 CFU/d vs 1.0×1010 CFU/d) and Saccharomycetes(1.0×106 CFU/d vs 1.0×108 CFU/d). After six weeks of treatment, glucose monitor was used to detect fasting blood-glucose and blood glucose at different time points. The content of glycosylated hemoglobin(HbA1 c) was detected with ELISA, and fecal microflora was detected using real-time fluorescence quantitative PCR. Results The levels of blood glucose and HbA1 c in low-and high-dose composite probiotics groups significantly reduced and oral glucose tolerance ameliorated compared with the model group. But there was no significant change in body weight. Compared with model group, the amount of Firmicutes, Actinobacteria and Escherichia coli obviously reduced while those of Bacteroidetes, Lactobacillus and Bifidobacterium increased in low-and high-dose composite probiotics groups. But there was no difference in total bacteria and Fusobacterium. Conclusion The composite probiotics isolated from fermented camel milk can improve the blood glucose by regulating the intestinal flora in db/db mice.