静脉血细胞悬液放置时间对小鼠血常规测定的影响

Effects of Storage Time on Blood Routine Test of Venous Haematocyte Suspension in Mice

目的 观察静脉血细胞悬液放置时间对小鼠血常规测定的影响。方法 随机选取成年ICR小鼠、C57BL/6 J小鼠各20只,采集定量静脉血液加到定量血细胞分析用稀释液中混匀,制成静脉血细胞悬液,于0 min、15 min、30 min、1 h、2 h、4 h、6 h、8 h在MEK-6410C全自动血细胞分析仪上采用微量血测定法测定血常规。结果 ICR小鼠和C57BL/6 J小鼠血常规数据变化趋势基本一致。与0 min相比,RBC在2 h、4 h、6 h和8 h均显著降低(P<0. 01),PLT在4 h、6 h和8 h均显著升高(P<0. 01),PCT在2 h、4 h、6 h和8 h均显著升高(P<0. 01),MPV在2 h、4 h、6 h和8 h均显著升高(P<0. 01),MCV在1 h、2 h、4 h、6 h和8 h均显著降低(P<0. 01),MCHC在30 min、1 h、2 h、4 h、6 h和8 h均显著升高(P<0. 01),MCH在1 h、2 h、4 h、6 h和8 h均显著升高(P<0. 01),HCT在1 h、2 h、4 h、6 h和8 h均显著降低(P<0. 01),WBC、RDW、PDW和HGB无显著变化(P>0. 05)。结论 微量血测定法测定小鼠血常规时,尽可能在制成静脉血细胞悬液后即刻完成。RBC总数在1 h内测定,与RBC相关的HCT和MCV指标测定不宜超过30 min,PLT测定在2 h完成,与PLT相关的PCT和MPV测定应在1 h内,WBC总数及HGB含量较稳定,可在8 h内完成。

Objective To investigate the effect of storage time on blood routine test of venous haematocyte suspension in mice.Method Twenty adult ICR mice and 20 C57BL/6 J mice were randomly selected,which were adopted venous blood.Collection each of quantitative venous blood added in diluent which were adequate for quantitative blood cell analytical instrument to be mixed to make venous haematocyte suspensions.Then detected by trace blood determination on 0 min,15 min,30 min,1 h,2 h,4 h,6 h,8 h in the MEK-6410C full-automatic blood cell analyzer.Result The change trend of blood routine data in ICR mice and C57BL/6 J mice is basically the same.Compared with 0 min,RBC decreased significantly at 2 h,4 h,6 h and 8 h(P<0.01),PLT increased significantly at 4 h,6 h and 8 h(P<0.01),PCT increased significantly at 2 h,4 h,6 h and 8 h(P<0.01),MPV increased significantly at 2 h,4 h,6 h and 8 h(P<0.01),MCV decreased significantly at 1h,2h,4h,6h and 8 h(P<0.01),MCHC increased significantly at 30 min,1h,2 h,4 h,6 h and 8 h(P<0.01),MCH increased significantly at 1h,2 h,4 h,6 h and 8 h(P<0.01),HCT decreased significantly at 1h,2 h,4 h,6 h and 8 h(P<0.01)and there were no significant changes in WBC,RDW,PDW and HGB(P>0.05).Conclusion By trace blood determination,the blood routine test of mice is done as soon as possible after the haematocyte suspension is made.The RBC is better to be measured in 1 h,HCT,MCV related to RBC were tested should not be more than 30 min.The PLT is better to be measured in 2 h,PCT,MPV related to PLT were tested should not be more than 1 h,The data of WBC and the content of HGB are stable and should be completed within 8 h.