氯化汞对中国大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响

Effects of Mercuric Chloride on the Expression of CATH-BF in the Skin and CATH-2 and CATH-3 in the Heart and Pancreas of Chinese Giant Salamander(Andrias davidianus)

为探讨氯化汞(HgCl 2)对大鲵皮肤CATH-BF基因及心脏和胰腺CATH-2、CATH-3基因表达的影响,将幼龄中国大鲵分别暴露于0(对照)、1、10、100 ng/L HgCl 2中24、48、72 h,采用实时定量RT-PCR方法分析HgCl 2处理不同时间后,大鲵皮肤CATH-BF、心脏和胰腺中CATH-2、CATH-3基因的表达情况。结果显示,与对照组相比,大鲵暴露于HgCl 224 h后,大鲵皮肤CATH-BF基因表达水平均呈现下降变化趋势,即1、100 ng/L HgCl 2对大鲵暴露24 h极显著下调了大鲵皮肤CATH-BF基因表达水平(P<0.01),10 ng/L HgCl 2对大鲵暴露24 h显著下调了大鲵皮肤CATH-BF基因表达水平(P<0.05),10、100 ng/L HgCl 2对大鲵暴露48 h显著诱导了大鲵皮肤CATH-BF基因表达(P<0.05),大鲵暴露于HgCl 272 h后,HgCl 2对大鲵皮肤CATH-BF基因表达无显著影响;大鲵暴露于HgCl 224 h后,不同质量浓度HgCl 2对大鲵心脏CATH-2基因表达水平有不同的影响,即1、10 ng/L对大鲵暴露24 h极显著下调了心脏CATH-2基因表达水平(P<0.01),100 ng/L HgCl 2对大鲵暴露24 h极显著上调了心脏CATH-2基因表达水平(P<0.01),10、100 ng/L HgCl 2对大鲵暴露48 h后显著下调了大鲵心脏CATH-2基因表达水平(P<0.05);10、100 ng/L HgCl 2对大鲵暴露24 h显著下调了大鲵胰腺CATH-2基因表达水平(P<0.05);1、10 ng/L HgCl 2对大鲵暴露24 h极显著诱导了心脏CATH-3基因表达水平(P<0.01),大鲵暴露于HgCl 224、48、72 h后,HgCl 2对胰腺CATH-3基因表达均无显著性影响。可见,HgCl 2对大鲵皮肤CATH-BF基因及胰腺和心脏CATH-2、CATH-3基因表达的影响处于动态变化模式,并且HgCl 2对大鲵胰腺和心脏CATH-2、CATH-3基因表达影响存在组织特异性差异。

In order to explore the effects of mercuric chloride(HgCl 2)on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander(Andrias davidianus),the juvenile stage giant salamanders were continuously exposed to 0(control),1,10,and 100 ng/L HgCl 2 for 24,48,72 h respectively,and the effects of mercuric chloride on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander were investigated by real time quantitative RT-PCR method.After exposure to HgCl 2 for 24 h,the expression level of CATH-BF in the skin of giant salamander was reduced compared with the control group.1 and 100 ng/L HgCl 2 very significantly down-regulated the expression of CATH-BF in the skin(P<0.01).10 ng/L HgCl 2 significantly down-regulated the expression of CATH-BF gene in the skin after exposure for 24 h(P<0.05).10 and 100 ng/L HgCl 2 significantly induced the expression of CATH-BF gene in the skin after exposure for 48 h(P<0.05).Exposure to HgCl 2 for 72 h had no significant effects on the expression of CATH-BF gene in the skin.Different concentrations of HgCl 2 had different effects on the expression of CATH-2 gene in the heart of the giant salamander.1 and 10 ng/L HgCl 2 significantly down-regulated the expression of CATH-2 gene in the heart after exposure for 24 h(P<0.01).100 ng/L HgCl 2 significantly up-regulated the expression of CATH-2 gene in the heart after exposure for 24 h(P<0.01).10 and 100 ng/L HgCl 2 significantly down-regulated the expression of CATH-2 gene in the heart of the giant salamander after 48 h of exposure(P<0.05).10 and 100 ng/L HgCl 2 significantly down-regulated the expression of CATH-2 gene in the pancreas after 24 h of exposure(P<0.05).1 and 10 ng/L HgCl 2 significantly induced the expression of CATH-3 gene in the heart after exposure to HgCl 2 for 24 h(P<0.01).HgCl 2 had no significant effect on the expression of CATH-3 gene in the pancreas after 24,48 and 72 h of exposure.It was concluded that the pattern of effects of mercuric chloride on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas of Chinese giant salamander was dynamic,and the effects of HgCl 2 on the expression of CATH-BF in the skin and CATH-2 and CATH-3 in the heart and pancreas showed tissue specificity.