期刊文献+

海洋弧菌鉴定与分类相关功能基因研究进展 被引量:4

Research Progress on Functional Genes Related to Identification and Classification of Vibrio spp.
下载PDF
导出
摘要 【目的】探究功能基因在海洋弧菌物种鉴定中的作用,为海洋弧菌多样性研究以及致病性弧菌的监测和防控提供参考。【方法】根据已有的弧菌分类学研究成果,阐述16S rRNA基因、管家基因和毒力基因等相关功能基因在海洋弧菌鉴定与分类研究中的应用进展。【结果】16S rRNA基因和功能基因可用于海洋弧菌的分类学及其快速检测鉴定的研究,对致病性弧菌的诊治具有重要的意义。【结论】功能基因和16S rRNA基因在海洋弧菌菌种鉴定中的广泛应用,提高了海洋弧菌种间分类的准确性,对海洋弧菌的多样性研究以及水产养殖和人类健康的风险评估有重要价值。探索新型功能基因在海洋弧菌分类学研究和快速鉴定中的应用有现实意义。 【Objective】To analyze the effect of functional genes in the identification of Vibrio spp.,and provide information for studying sea Vibrio variety,and detecting and preventing marine pathogenic Vibrio.【Method】The application of related functional genes such as 16S rRNA genes,housekeeping genes and virulence genes in the identification and classification of Vibrio spp.were reviewed.【Result】16S rRNA genes and functional genes can be used in the rapid detection and identification of Vibrio spp.,which are of great significance for the diagnosis and treatment of pathogenic Vibrio.【Conclusion】The wide applications of functional genes and 16S rRNA genes improved the accuracy of interspecific classification of Vibrio spp.,and were important for the study of the Vibrio spp.diversity and the risk assessment of aquaculture and human health.In addition,it is still necessary to explore the application potential of new functional genes in the taxonomy and the rapid identification of Vibrio spp.
作者 陈星 唐金利 姜宫凌侠 亢振军 李楠 CHEN Xing;TANG Jin-li;JIANG Gong-ling-xia;KANG Zhen-jun;LI Nan(Key Laboratory of Environment Change and Resource Use in Beibu Gulf,Nanning normal University,Nanning 530001,China;School of Life Science and Technology,Guangxi University,Nanning 530004,China;School of Oceanography,Beibu Gulf University,Qinzhou 535011,China)
出处 《广东海洋大学学报》 CAS 2020年第1期116-125,共10页 Journal of Guangdong Ocean University
基金 广西自然科学基金项目(2017GXNSFAA198081,2018GXNSFDA281006,2016GXNSFBA380108) 国家自然科学基金项目(41966005) 广西高校引进海外高层次人才百人计划项目(6020303891251) 北部湾环境演变与资源利用教育部重点实验室(南宁师范大学)系统基金(GTEU-KLOP-X1701,GTEU-KLOP-X1806,GTEU-KLXTJJ-201717) 广西“海洋生态环境”院士工作站能力建设项目(桂科AD17129046)
关键词 海洋弧菌 功能基因 分类鉴定 16S rRNA基因 Vibrio spp. functional genes identification and classification 16S rRNA gene
  • 相关文献

参考文献6

二级参考文献101

  • 1Sambrook J Fritsch EF 等.分子克隆(第二版)[M].北京:科学出版社,1992.956.
  • 2祁国明 中华人民共和国卫生产卫生防疫司.埃尔托乱弧菌两类菌株肠毒素原性的比较研究.副霍乱资料汇编[M].北京:中华流行病学杂志编辑部,1984.294-303.
  • 3于永茂 刘延清 等.霍乱A^-B^+减毒活菌苗的构建[J].中华流行病学杂志,1993,14(15):136-141.
  • 4Dunlap PV, Tsukamoto KK. Luminous Bacteria. Prokaryotes, 2006(2): 863-892.
  • 5Hwang ET, Lee JH, Chae YJ, et al. Analysis of the toxic mode of action of silver nanopartieles using stress-specific bioluminescent bacteria. Small, 2008, 4(6): 746-750.
  • 6Ahn JM, Hwang ET, Youn CH. Prediction and classification of the modes of genotoxic actions using bacterial biosensors specific for DNA damages. Biosensors and Bioelectronics, 2009, 25(4): 767-772.
  • 7Golding GR, Kelly CA, Sparling R, et al. Evaluation of mercury toxicity as a predictor of mercury bioavailability. Environ Sci Technol, 2007, 41(16): 5685-5692.
  • 8Li YF, Li FY, Ho CL, et al. Construction and comparison of fluorescence and bioluminescence bacterial biosensors for the detection of bioavailable toluene and related compounds. Environmental Pollution, 2008, 52(1): 123-129.
  • 9Leedjarv A, Ivask A, Virta M, et al. Analysis of bioavailable phenols from natural samples by recombinant luminescent bacterial sensors. Chemosphere, 2006, 64(11): 1910-1919.
  • 10Lee JH, Mitchell RJ, Gu MB. Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa::luxCDABE). Journal of Biotechnology, 2007, 131(3): 330-334.

共引文献59

同被引文献96

引证文献4

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部