摘要
【目的】探究功能基因在海洋弧菌物种鉴定中的作用,为海洋弧菌多样性研究以及致病性弧菌的监测和防控提供参考。【方法】根据已有的弧菌分类学研究成果,阐述16S rRNA基因、管家基因和毒力基因等相关功能基因在海洋弧菌鉴定与分类研究中的应用进展。【结果】16S rRNA基因和功能基因可用于海洋弧菌的分类学及其快速检测鉴定的研究,对致病性弧菌的诊治具有重要的意义。【结论】功能基因和16S rRNA基因在海洋弧菌菌种鉴定中的广泛应用,提高了海洋弧菌种间分类的准确性,对海洋弧菌的多样性研究以及水产养殖和人类健康的风险评估有重要价值。探索新型功能基因在海洋弧菌分类学研究和快速鉴定中的应用有现实意义。
【Objective】To analyze the effect of functional genes in the identification of Vibrio spp.,and provide information for studying sea Vibrio variety,and detecting and preventing marine pathogenic Vibrio.【Method】The application of related functional genes such as 16S rRNA genes,housekeeping genes and virulence genes in the identification and classification of Vibrio spp.were reviewed.【Result】16S rRNA genes and functional genes can be used in the rapid detection and identification of Vibrio spp.,which are of great significance for the diagnosis and treatment of pathogenic Vibrio.【Conclusion】The wide applications of functional genes and 16S rRNA genes improved the accuracy of interspecific classification of Vibrio spp.,and were important for the study of the Vibrio spp.diversity and the risk assessment of aquaculture and human health.In addition,it is still necessary to explore the application potential of new functional genes in the taxonomy and the rapid identification of Vibrio spp.
作者
陈星
唐金利
姜宫凌侠
亢振军
李楠
CHEN Xing;TANG Jin-li;JIANG Gong-ling-xia;KANG Zhen-jun;LI Nan(Key Laboratory of Environment Change and Resource Use in Beibu Gulf,Nanning normal University,Nanning 530001,China;School of Life Science and Technology,Guangxi University,Nanning 530004,China;School of Oceanography,Beibu Gulf University,Qinzhou 535011,China)
出处
《广东海洋大学学报》
CAS
2020年第1期116-125,共10页
Journal of Guangdong Ocean University
基金
广西自然科学基金项目(2017GXNSFAA198081,2018GXNSFDA281006,2016GXNSFBA380108)
国家自然科学基金项目(41966005)
广西高校引进海外高层次人才百人计划项目(6020303891251)
北部湾环境演变与资源利用教育部重点实验室(南宁师范大学)系统基金(GTEU-KLOP-X1701,GTEU-KLOP-X1806,GTEU-KLXTJJ-201717)
广西“海洋生态环境”院士工作站能力建设项目(桂科AD17129046)
关键词
海洋弧菌
功能基因
分类鉴定
16S
rRNA基因
Vibrio spp.
functional genes
identification and classification
16S rRNA gene