狼爪瓦松愈伤组织生物反应器培养及提高有效物质积累研究

Callus bioreactor culture of Orostachys cartilaginea A. Ber and improvement of effective compounds accumulation

为筛选狼爪瓦松愈伤组织生物反应器培养的适宜接种量,该试验研究了4种接种量(10.0、12.5、15.0、17.5 g/L)对愈伤组织生长和有效物质积累的影响。结果表明:随着接种量的增加,愈伤组织生物量随之升高,在接种量为15 g/L时,狼爪瓦松愈伤组织中总黄酮、总酚及总多糖的含量和生产量达到了最大值。另外,为促进愈伤组织中有效物质的积累,以茉莉酸甲酯(MeJA)作为诱导子,在愈伤组织培养25 d后用不同浓度的MeJA处理。结果发现MeJA处理对愈伤组织中有效物质的积累有促进作用,当MeJA浓度为100μmol/L时,狼爪瓦松愈伤组织中黄酮、总酚和总多糖的含量及生产量达到最大。

To select a suitable inoculation density in callus bioreactor culture of Orostachys cartilaginea,the present study designed four inoculation(10.0,12.5,15.0,17.5 g/L)densities and investigated inoculation density on biomass and effective compound accumulation of callus.The results showed that biomass of callus increased with increasing the inoculation density,the highest contents and productivities of flavonoids,phenolics,and polysaccharides in callus were determined at inoculation density of 15 g/L.In addition,to promote effective compound accumulation,methyl jasmonate(MeJA)was used as an elicitor to treat 25-day-old bioreactor cultured callus.The MeJA treatment with different concentrations could enhance effective compound accumulation,the maximum content and productivities of flavonoids,phenolics,and polysaccharides in callus were found when callus were treated with 100μmol/L MeJA for 2 days.