磁性纳米颗粒负载质粒DNA的研究

Study on magnetic nanoparticle loading plasmid DNA

【目的】研究磁性纳米颗粒(MNP)负载质粒DNA形成的纳米载体−基因复合物的生物物理学特性、负载形态与负载机理,为基于磁性纳米载体定向编辑技术的应用奠定基础。【方法】以MNP作为基因载体,与质粒DNA pRGEB32制备纳米载体−基因复合物MNP−pRGEB32,分析MNP负载、保护pRGEB32的能力,并对其粒度分布、Zeta电位、结合形态进行研究。【结果】MNP通过静电作用压缩、吸附、聚集pRGEB32,形成纳米载体−基因复合物。MNP能有效负载并保护pRGEB32。【结论】MNP可作为一种理想的基因转移载体。

【Objective】To study biophysical property,loading morphology and loading mechanism of nanocarrier-gene complex prepared by magnetic nanoparticle(MNP)loading plasmind DNA,and lay a foundation for application of oriented editing technology based on magnetic nano carrier.【Method】MNP was used as gene carrier to prepare nanocarrier-gene complex MNP-pRGEB32 with plasmid DNA pRGEB32.The abilities of MNP loading and protecting pRGEB32 were analyzed.The particle size distribution,Zeta potential and loading morphology of MNP-pRGEB32 were investigated.【Result】MNP compressed,adsorbed and aggregated pRGEB32 through electrostatic interaction to form nanocarrier-gene complex.MNP could effectively load and protect pRGEB32.【Conclusion】MNP can be used as an ideal gene transfer carrier.