深海链霉菌Streptomyces sp.PKU-MA01297中发现的两个葡萄糖苷化角环素（英文）

Two glucosylated angucyclines from deep-sea-derived Streptomyces sp.PKU-MA01297

角环素类天然产物是由放线菌中发现的由Ⅱ型聚酮合酶(PKS)产生的芳香聚酮化合物。它们的结构中不同位置的糖基团取代扩展了它们的结构多样性。本文报道了从一株深海链霉菌Streptonlyces sp.PKU01297中发现的两个新的角环素类化合物(化合物1和2)。链霉菌Streptomyces sp.PKU01297分离自印度洋3202米深的海底沉积物。化合物1和2的结构通过综合分析NMR,MS和CD等实验数据得到解析,它们结构中的1-O-β-D-吡喃葡萄糖基团为首次在角环素类化合物中发现,丰富了深海天然产物的结构类型。针对化合物1和2开展了抗菌、抗肿瘤细胞毒和抗炎活性筛选,未发现两个化合物具有相关生物活性。本文的工作为将来开展化合物1和2的生物合成研究和更多的生物活性测试打下了基础。

Angucyclines are aromatic polyketides produced by typeⅡpolyketide synthase(PKS)exclusively from actinomycetes.These natural products contain hydrolyzable sugar moieties that attach to various positions of the polyketide skeleton and expand the structural diversity.We here report the isolation of two new angucyclines(1 and 2)from the deep-sea-derived Streptomyces sp.PKU-MA01297,which was isolated from a sediment sample collected at a depth of 3202 meters from the Indian Ocean.The structures of the two new compounds were elucidated based on comprehensive methods,including NMR,MS and CD analysis.Compounds 1 and 2 featured a 1-O-β-D-glucopyranosyl moiety that has not been reported for angucyclines.Several biological activity assays,including antibacterial,cytotoxicity and anti-inflammatory assays,were carried out,and compounds 1 and 2 showed no activities.These results set the stage for biosynthetic research and more biological activity assays in the future.