驼乳清蛋白影响糖尿病大鼠胰腺β细胞功能的效果以及作用机制初探

Study on the effect of camel whey protein on pancreatic β cell function and its mechanism in rats with diabetes

目的观察驼乳清蛋白对于糖尿病模型大鼠胰腺β细胞功能的影响,并初步分析驼乳清蛋白的作用机制。方法选取实验用Wistar SPF级300~800 g的雄性大鼠10只以链尿佐菌素(STZ)诱发法建立糖尿病大鼠模型,分离筛选胰腺β细胞后进行体外培养,将以胎牛血清RPMI-1640作为培养基的胰腺β细胞作为模型对照组2只,将以乳清蛋白作为培养基的胰腺β细胞作为实验组3只,同时分离筛选正常大鼠的胰腺β细胞进行体外培养(空白对照组5只),应用MTT比色法对各组胰腺β细胞生长曲线进行绘制,并在干预后24 h、48 h、72 h、96 h分别进行葡萄糖依赖性促胰岛素释放肽(GIP)、胰岛素样生长因子-1(IGF-1)、胰高血糖素样多肽1(GLP-1)、白细胞介素-1α(IL-1α)以及超氧化物歧化酶(SOD)水平测定。结果①相同时间点,空白对照组胰腺β细胞贴壁率、细胞相对增殖速度GIP(78.50±1.10)pmol/L、IGF-1(38.65±3.25)μg/L、GLP-1(258.30±2.65)pmol/L、SOD(38.65±3.25)U/ml水平显著高于模型对照组和实验组(P<0.05),IL-1α(38.65±2.65)pg/ml显著低于模型对照组和实验组(P<0.05);②相同时间点,实验组胰腺β细胞贴壁率、细胞相对增殖速度GIP(60.45±1.89)pmol/L、IGF-1(164.76±2.82)μg/L、GLP-1(31.56±2.32)pmol/L、SOD(42.25±2.85)U/ml显著高于模型对照组(P<0.05),IL-1α(40.03±2.76)pg/ml显著低于模型对照组(P<0.05)。结论驼乳清蛋白能够促进胰腺β细胞生长,其可能成为2型糖尿病防治的新方法。

Objective To observe the effect of camel whey protein on pancreaticβcell function in rats,and to analyze the mechanism of the action of camel whey protein.Methods 10 male rats with Wistar SPF grade of about 300 to 800 g were selected.The diabetic rat model was established by Streptozotocin(STZ),and the pancreaticβcells were isolated and cultured in vitro.Pancreaticβcells with fetal bovine serum RPMI-1640 was model control group(2 cases),the pancreaticβcells with whey protein was the experimental group(3 cases),and the pancreaticβcells of normal mice were isolated and cultured in vitro(blank control group,5 cases).MTT colorimetric method was used to plot the growth curve ofβ-cells in each group.After the intervention for 24 h,48 h,72 h,96 h,the content of glucose dependent insulin releasing peptide(GIP),insulin like growth factor(IGF-1),Glucagon like peptide 1(GLP-1),Interleukin 1 alpha(IL-1α),and superoxide dismutase(SOD)were measured.Results①At the same time point,in the blank control group,the adherence rate ofβcells,the relative proliferation rate of P(78.50±1.10)pmol/L,IGF-1(38.65±3.25)μg/L,GLP-1(258.30±2.65)pmol/L,SOD(38.65±3.25)U/ml were significantly higher than those in the model control group and the experimental group(P<0.05),and the IL-1α(38.65±2.65)pg/ml was significantly lower than that of the model control group and the experimental group(P<0.05).②At the same time point,in the experimental group,the adherence rate ofβcells,the relative proliferation rate of GIP(60.45±1.89)pmol/L,IGF-1(164.76±2.82)μg/L.GLP-1(31.56±2.32)pmol/L.SOD(42.25±2.85)U/ml were significantly higher than those in the model control group(P<0.05),and the IL-1α(40.03±2.76)pg/m was significantly lower than that in the model control group(P<0.05).Conclusion Camel whey protein can promote the growth of pancreaticβcells,which may be a new method for the prevention and treatment of type 2 diabetes.