两种消毒方法对不同介质上细菌芽孢杀灭效果比较

Comparison of the killing effects of two disinfection methods on bacterial spores on different media

目的比较饱和过氧乙酸熏蒸和汽化过氧化氢消毒机消毒对生物安全Ⅲ级实验室内不同种类介质上细菌芽孢的杀灭效果。方法采用载体定量杀菌试验和理化分析方法,对两种消毒方法杀灭生物安全Ⅲ级实验室内不同性质载体表面枯草杆菌黑色变种芽孢的效果进行观察。结果饱和过氧乙酸熏蒸和汽化过氧化氢消毒机对放置在生物安全Ⅲ级实验室内不同位置的布片、金属片、滤纸片、玻片和蛋壳上污染的枯草杆菌黑色变种芽孢的杀灭对数值均>5.00。饱和过氧乙酸对铜片和碳钢片有中度腐蚀,对铝片有轻度腐蚀,对不锈钢片基本无腐蚀;汽化过氧化氢消毒对铜片、碳钢片、铝片、不锈钢片均基本无腐蚀。结论两种消毒方法对不同物体表面消毒均有良好的消毒效果,饱和过氧乙酸熏蒸法对金属的腐蚀性较汽化过氧化氢强。

Objective To compare the killing effect of saturated peracetic acid fumigation and vaporized hydrogen peroxide disinfection machine on bacterial spores on different types of media in biosafety levelⅢlaboratory.Methods The carrier quantitative bactericidal test and physical and chemical analysis method were used to observe the effect of two disinfection methods on killing spores of Bacillus subtilis var.niger on different carriers of biosafety levelⅢlaboratory.Results The killing logarithm values of saturated peracetic acid fumigation and vaporization hydrogen peroxide disinfection machine to the contaminated spores of Bacillus subtilis var.niger on the cloth,metal sheets,filter paper,slides and eggshells placed in different positions of biosafety levelⅢlaboratory were all>5.00.Saturated peracetic acid had moderate corrosion on copper and carbon steel sheets,mild corrosion on aluminum sheets,and no corrosion on stainless steel sheets;vaporized hydrogen peroxide disinfection nearly had no corrosion on copper sheets,carbon steel sheets,aluminum sheets and stainless steel sheets.Conclusion The two disinfection methods have good disinfection effect on the surface of different objects.The saturated peracetic acid fumigation method is more corrosive to metal than vaporized hydrogen peroxide.