不同LED光质对石蒜幼苗生长、生理和生物碱积累的影响

Effects of the quality of LED light on the growth, physiological characteristics, and the accumulation of alkaloids in Lycoris radiata

为通过环境调控提高石蒜产量、增加生物碱含量,设置6种光质,即白光(CK)、红光(R)、蓝光(B)以及红蓝复合光RB(1:2)、RB(2:1)、RB(1:1),研究不同光质对石蒜幼苗生长、生理特性和生物碱积累的影响,筛选适合石蒜幼苗生长和生物碱积累的光质条件.结果表明:与CK相比,红蓝复合光质可以加快促进石蒜幼苗的生长,其中进行红蓝复合光RB(1:2)处理后,根长、根数、干重、鲜重和鳞茎直径等都明显比CK更高,依次提高了10.91%、146.48%、158.69%、34.92%和133.33%;单一的红光不利于石蒜幼苗叶绿素的合成,一定比例的红蓝复合光能够促进叶绿素合成,RB(1:2)处理后幼苗叶绿素含量达到最高值,比CK约高出1.22倍;而RB(2:1)处理后,促进了石蒜幼苗可溶性糖的积累,蓝光能够提高其可溶性蛋白的含量;与CK相比,RB(1:2)处理下石蒜幼苗丙二醛含量显著降低,超氧化物歧化酶和过氧化物酶活性较低,说明RB(1:2)处理可以显著降低膜脂过氧程度;B处理下石蒜幼苗加兰他敏、石蒜碱和力可拉敏含量最高,分别是CK的1.84倍、1.62倍和1.11倍.可以看出,RB(1:2)处理对石蒜幼苗的生长、叶片光合色素合成和生物量积累都起到了积极的促进作用,并且膜脂过氧化程度比较低,因此是石蒜幼苗的最佳生长光质条件,B处理(蓝光)可以促进石蒜生物碱的积累;结果可为利用LED光质调控技术提高石蒜种苗的质量提供理论基础.(图2表3参33)

We conducted this experiment to explore the effects of LED light quality on the growth,physiological characteristics and accumulation of alkaloids in Lycoris radiata seedlings.We hope that identifying the optimal light conditions will help maximize growth and the production of alkaloids through environmental regulation.L.radiata seedlings were cultured under different growing diodes(LEDs),including white light(CK),blue light(B),red light(R),and red and blue composite lights RB(1:2),RB(1:1),RB(2:1)to analyze the effects of LED light quality on the growth and physiological characteristics of L.radiata seedlings.The results showed that the growth indices of L.radiata seedlings under red and blue composite lights(RB 1:2)were improved compared to growth under CK.Following RB(1:2)exposure,the plant fresh weight,plant dry weight,root length,root number,and bulb diameter were significantly increased by 158.69%,133.33%,10.91%,146.48%,and 34.92%,respectively,compared to exposure to CK.R exposure had a negative effect on the biosynthesis of chlorophyll,while a certain proportion of red and blue composite light could promote chlorophyll synthesis.Exposure to RB(1:2)composite light produced the highest chlorophyll content,which was 1.22 times higher than the amount of chlorophyll produced following exposure to CK.RB(2:1)exposure promoted the accumulation of soluble sugar in L.radiata seedlings,and blue light increased the content of soluble protein in the seedlings.Compared with CK exposure,the maleicdialdehyde(MDA)content,superoxide dismutase(SOD)and peroxidase(POD)activities in L.radiata seedlings under RB(1:2)treatment were significantly lower,thus indicating that RB(1:2)treatment could significantly reduce membrane lipid peroxidation.B exposure yielded the highest levels of galanthamine,lycorine,and lycoramine in L.radiata seedlings,which were 1.84,1.62,and 1.11 times higher than those after CK exposure,respectively.In general,RB(1:2)exposure was beneficial for the growth,accumulation of biomass,increasing the synthesis of photosynthetic pigments in L.radiata seedlings,and reducing membrane lipid peroxidation.RB(1:2)provided the optimal light quality for the growth of L.radiata seedlings,while B treatment optimized the production of alkaloids.This study provided a theoretical foundation and technical support for the modulation of LED light quality for the production of high-quality L.radiata seedlings.