Trichoderma sp.WL-Go细胞提取物合成纳米金影响因素及其催化特性

Influencing factors and catalytic characteristics of gold nanoparticles biosynthesized with the cell-free extracts of Trichoderma sp. WL-Go

与传统的物理和化学合成方法相比而言,生物法合成纳米金具备环境友好、反应条件温和、低毒等优势,近年来受到了广泛关注.利用真菌Trichoderma sp.WL-Go细胞提取物合成纳米金,探究氯金酸浓度、pH、温度等反应条件对纳米金合成的影响,最后对生物纳米金催化还原4-硝基苯酚的性能进行了考察.结果显示,氯金酸浓度、pH以及反应温度对纳米金的合成具有重要影响,影响主要体现在合成速率以及纳米金稳定性方面.温度的提高可加速纳米金合成,通过TEM分析可知30℃和60℃条件下合成的纳米金平均粒径分别为15.0 nm和15.1 nm,均为球形和伪球形.对4-硝基苯酚的催化还原试验表明,在60℃条件下合成的纳米金催化速率优于30℃条件下合成的纳米金.本研究表明利用菌株Trichoderma sp.WL-Go细胞提取物可以合成尺寸均一且分散性良好的纳米金颗粒,且温度的提高可在加速纳米金合成的同时提升其催化活性,对生物合成纳米金的工业化应用有一定积极意义.(图4表1参23).

Compared to physical or chemical synthesis methods,the biosynthesis of gold nanoparticles(AuNPs)has recently attracted extensive attention owing to its higher environmentally friendly nature,milder reaction conditions,and lower toxicity effects.In this study,cell-free extracts of Trichoderma sp.WL-Go were used to synthesize AuNPs.The effects of chloroauric acid concentration,pH,and temperature on the biosynthesis process were investigated,and the catalytic ability of as-synthesized AuNPs to reduce 4-nitrophenol was discussed.Based on our results,chlorometallic acid concentration,pH,and temperature had important effects on the synthesis of AuNPs,which were mainly reflected in the synthesis rate and stability of the AuNPs.An increase in temperature could accelerate the synthesis process and through TEM,the average particle size of the AuNPs synthesized at 30℃and 60℃was found to be 15.0 nm and 15.1 nm,respectively,with both spherical and pseudospherical appearances.According to the catalytic experimental results of the 4-nitrophenol reduction,the catalytic rate was higher for AuNPs synthesized at 60℃than at 30℃.In conclusion,this study shows that the cell-free extracts of Trichoderma sp.WL-Go could be used to synthesize AuNPs with good dispersion and uniform size.Furthermore,an increase in temperature can accelerate the synthesis of AuNPs and enhance their catalytic activity,which have a positive significance in the industrial application of biosynthesized AuNPs.