聚花野丁香的化学成分及其抗氧化活性

Chemical components from Leptodermis glomerata and their antioxidant activity

为研究聚花野丁香(Leptodermis glomerata)的生物活性,综合利用多种色谱方法,对该植物95%乙醇提取物的化学成分进行了分离、纯化,从中共得到15个化合物.通过波谱数据分析及比对,将其分别鉴定为杨梅素(1)、(2R,3R)-二氢杨梅素(2)、槲皮素(3)、pinostrobin(4)、1,3-二甲氧基-2-羟基蒽醌(5)、2-甲氧基-1,3-二羟基蒽醌(6)、1,3,4-三甲氧基-2-羟基蒽醌(7)、1,2,3,4-四甲氧基蒽醌(8)、1,2,3-三甲氧基蒽醌(9)、5,7-二羟基色原酮(10)、3S-faramol(11)、(2R,4S)-catalponol(12)、(2S,4S)-catalponol(13)、3β-乙酰基齐墩果酸(14)、齐墩果酸(15).采用DPPH、ABTS、NO三种自由基清除活性测试模型,对上述化合物的抗氧化活性进行测试.结果表明,(2R,3R)-二氢杨梅素(2)、pinostrobin(4)和5,7-二羟基色原酮(10)表现出显著的自由基清除活性:当浓度为1 mg/mL时,化合物2对DPPH、NO自由基的清除率为91.2%、67.8%;当浓度为2 mg/mL时,化合物4对DPPH自由基的清除率为102.8%;当浓度为1 mg/mL时,化合物10对ABTS、NO自由基的清除率分别为104.7%、95.7%.本研究表明聚花野丁香富含多羟基酚类化合物,其中部分化合物显示较强的抗氧化活性,具有开发利用价值.(图1表1参23)

Fifteen natural products were isolated from the aqueous ethanol extract of Leptodermis glomerata using various chromatographic methods.Based on spectrum data,these compounds were identified as follows:myricetin(1),(2R,3R)-dihydromyricetin(2),quercetin(3),pinostrobin(4),1,3-demethoxy-2-hydroxyanthraquinone(5),2-methoxy-1,3-dehydroxyanthraquinone(6),1,3,4-trimethoxy-2-hydroxyanthraquinone(7),1,2,3,4-tetramethoxy anthraquinone(8),1,2,3-trimethoxyanthraquinone(9),5,7-dihydroxyprimary ketone(10),3S-faramol(11),(2R,4S)-catalponol(12),(2S,4S)-catalponol(13),3β-acetyloleanolic acid(14),and oleanolic acid(15).(2R,3R)-Dihydromyricetin(2),pinostrobin(4),and 5,7-dihydroxyprimaryketone(10)exhibited significant radical scavenging effects according to the results of 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2′-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid(ABTS),and nitric oxide(NO)activity assays.Compound 2(1 mg/mL)showed 91.2%and 67.8%radical scavenging activities against DPPH and NO radicals,respectively.Compound 4(2 mg/mL)showed 102.8%DPPH radical scavenging activity.Compound 10(1 mg/mL)was able to scavenge ABTS and NO radicals with 104.7%and 95.7%radical scavenging activity,respectively.