绿花百合组培芽增殖与试管鳞茎膨大的影响因素研究

Study on the Influencing Factors of Bud Proliferation and Bulblet Swelling of Lilium fargesii in vitro

为优化绿花百合组培芽增殖与试管鳞茎膨大的条件,分析了培养基中蔗糖含量、激素浓度及其配比、硫酸腺嘌呤及水解酪蛋白对绿花百合组培芽增殖的影响,以及蔗糖含量、激素浓度及其配比对试管鳞茎膨大的影响。结果表明:在(23±1)℃和12 h/d光照培养条件下,MS培养基附加0.2 mg/L TDZ、0.2 mg/L NAA、30 mg/L硫酸腺嘌呤、200 mg/L水解酪蛋白和30 g/L蔗糖,有利于绿花百合增殖,增殖系数为5.59;适宜绿花百合鳞茎膨大的最佳培养基为MS附加0.5 mg/L 6-BA、0.3 mg/L NAA及60 g/L蔗糖。因此,蔗糖浓度及温度过高或过低均不利于绿花百合组培芽增殖,光培养下绿花百合组培芽增殖系数高于暗培养,添加适宜浓度的水解酪蛋白对不定芽的分化有良好的促进作用,并且细胞分裂素和生长素协同作用对组培芽增殖和鳞茎膨大有显著的促进作用。

In order to optimize the factors affecting the bud proliferation and bulblet swelling of Lilium fargesii in vitro.The effects of sucrose concentration,hormone concentrations and sucrose combinations,concentrations of adenine sulphate and casein hydrolysate on the proliferation,and sucrose concentration,hormone concentrations and combinations on the swelling in vitro were investigated.The results showed that MS+TDZ 0.2 mg/L+NAA 0.2 mg/L+Sucrose 30 g/L+Adenine sulphate 30 mg/L+Casein hydrolysate 200 mg/L was suitable for bud proliferation from scales of L.fargesii in vitro under the condition of the culture temperature of(23±1)℃and light culture of 12h/d,and multiplication coefficient was 5.59;The optimal medium of bulblet swelling was MS+NAA 0.3 mg/L+6-BA 0.5 mg/L+Sucrose 60 g/L.This study comes a conclusion that excessive or too low concentration of sucrose was to the disadvantage of the bud proliferation and swelling of test-tube bulblets of L.fargesii,the multiplication rate in light was higher than in dark,suitable combinations of casein hydrolysate played an auxoaction for the bud proliferation and bulblet swelling,and synergism of cytokinin and auxin can significantly promote the bud proliferation and bulblet swelling.