美国车厘子组织培养试验

Cerasus pseudocerasus Tissue Culture Test

采用美国思加图车厘子室内盆栽萌发的嫩芽和老芽作为外植体,分析不同处理时间对车厘子外植体萌芽效果的影响;采用不同浓度的植物生长调节剂对其进行诱导增殖和生根培养,筛选最佳组培方法。结果表明:嫩芽是车厘子外植体的最佳选择,通过7 s酒精和0.1%升汞9 min消毒获得无菌体,污染率低、褐化率较低、萌芽率60%。芽诱导培养基为MS+6-BA 1.0 mg/L+NAA 0.2 mg/L,增殖系数为3.92。生根培养基为1/2MS+NAA0.6 mg/L,生根率为100%。移栽炼苗基质比例为V(腐殖土)∶V(红土)=4∶6,盖膜驯化,保湿温度(25±2)℃,湿度80%,成活率达90%以上。

The old branches and branches of new germination buds in indoor potted plants in the spring of Cerasus pseudocerasus were selected as explants.The effect of hormone treatment time on the induction of cluster buds were studied.Different concentrations of 6-BA,NAA,IAAand IBA were used to induce proliferation and rooting of cherry in the study.The results showed that the buds were the best choice for cherry explants.The explants were sterilized with 75%alcohol for 7 s and 0.1%corrosive sublimate for 9 min,with low pollution rate,low browning rate and 60%germination rate.The shoot induction medium was Murashige and Skoog medium(MS)with 1.0 mg/L 6-benzylaminopurine(6-BA)and 0.2 mg/L 1-naphthlcetic acid(NAA),the proliferation coefficient attained 3.92.The rooting medium contained 1/2 MS with 0.6 mg/L NAA,and the rooting rate was 100%.The proportion of substrate for transplanting and curing seedlings was 40%humus and 60%red clay,the temperature(with a moisture cover film)was 25±2℃,the humidity was 80%,and the survival rate was more than 90%.