摘要
[目的]以大葱花序轴作为外植体材料,研究不同培养基组成对大葱花序轴愈伤组织诱导、芽分化、继代和生根培养的影响。[方法]以花蕾作为外植体,采用不同浓度的6-BA和NAA组合配成8种MS培养基,筛选出诱导、分化、继代和生根培养较适合的培养基。[结果]MS+BA7.0 mg/L+NAA 0.01 mg/L对愈伤诱导的效果最好;MS+6-BA 0.5 mg/L+NAA 0.01 mg/L为分化培养基的适宜配方;MS+6-BA 0.4 mg/L+NAA 0.2 mg/L为继代培养基的适宜配方;MS+IAA 0.05 mg/L生根效果很好。[结论]为大葱花序轴组织培养提供参考依据。
[Objection]The aim was to study on tissue culture of welsh onion from rachis.[Method]The flower bud was used as the explant with eight different concentration mediums of 6-BA and NAA.The adaptive medium was screened of induction,differentiation and rooting culture.[Result]The suitable culture medium for rachis regeneration was MS+BA 7.0 mg/L+NAA 0.01 mg/L.MS+BA 0.5 mg/L+NAA0.1 mg/L was suitable formulations for differentiation medium.MS+BA 0.4 mg/L+NAA 0.2 mg/L was suitable formula for subculture medium.MS+IAA 0.05 mg/L was suitable for rooting well.[Conclusion]The study could provide references for welsh onion bud tissue culture.
作者
李娜
贾俊香
杨国栋
姜滨滨
蒋欣陶
田云
张艳玲
崔连伟
LI Na(Vegetables Research Institute,Liaoning Academy of Agriculture Sciences,Shenyang,Liaoning 110161)
出处
《园艺与种苗》
CAS
2019年第12期1-2,58,共3页
Horticulture & Seed
基金
国家特色蔬菜产业技术体系-大葱品种改良岗位项目（CARS-24-A-08）
关键词
大葱
花序轴
组织培养
Welsh Onion
Rachis
Tissue culture
