竹节参多糖对对乙酰氨基酚诱导的小鼠肝损伤的保护作用

Protective effects of polysaccharides from Panax japonicus on mice with liver injury induced by acetaminophen

目的:探究竹节参多糖(PSPJ)对对乙酰氨基酚(APAP)诱导的小鼠肝损伤的保护作用.方法:将32只小鼠随机分为对照组、模型组、PSPJ低(50 mg·kg^-1)、中(100 mg·kg^-1)、高(200 mg·kg^-1)剂量组,腹腔注射APAP(400 mg·kg^-1)建立肝损伤模型.通过检测实验小鼠的存活率,肝脏病理变化,血清中AST、ALT、LDH的水平,肝脏的GSH、MDA水平及炎症因子IL-1β、IL-6浓度变化情况来观察竹节参多糖对APAP诱导的小鼠急性肝损伤的保护作用.结果:在连续7天灌胃给予竹节参多糖之后,小鼠生存率提高(P<0.05或P<0.01),肝脏病理变化明显缓解;竹节参多糖还显著降低了肝损伤小鼠血清中ALT、AST、LDH及炎症因子IL-1β和IL-6水平(P<0.05或P<0.01),增加了肝组织中GSH的水平并下调了MDA的含量(P<0.05或P<0.01).结论:竹节参多糖可能通过抗氧化和抗炎途径来减缓APAP诱导的急性肝损伤.

Objective: To study the protective effects of polysaccharides from Panax japonicus(PSPJ) on N-acetyl-para-aminophenol(APAP) induced liver injury in mice. Methods: 32 mice were randomly divided into control group,model group,low, medium and high PSPJ dose groups( 50, 100 and 200 mg·kg^-1),and mouse acute liver injury model were established by intraperitoneal injection of 400 mg·kg^-1 APAP.The survival rate, liver pathological changes, serum AST, ALT and LDH levels, liver GSH and MDA levels, and changes in IL-1β and IL-6 concentrations of the experimental mice were measured to study the protective effect of PSPJ on APAP induced acute liver injury. Results: After 7 consecutive days of oral administration of PSPJ, the survival rate of mice was improved and the pathological changes in liver were relieved(P<0.05 or P<0.01). Compared with the model group,PSPJ significantly declined the levels of ALT, AST, LDH and inflammatory factors IL-1β, IL-6 in serum(P<0.05 or P<0.01), increased the hepatic level of GSH and reduced the contents of MDA(P<0.05 or P<0.01). Conclusion: PSPJ has protective effect on APAP induced liver injury in mice, and the mechanism may be related to its anti-oxidation and anti-inflammatory properties.