响应面法优化番木瓜籽蛋白质提取工艺

Optimization of Carica papaya L. Seed Protein Extraction Using Response Surface Methodology

作为食用和工业生产的废弃物番木瓜籽,提高其蛋白质提取率,实现资源的回收利用和扩大副产品价值。以番木瓜籽为原料,使用响应面法优化碱溶酸沉法提取番木瓜籽蛋白质提取工艺。以番木瓜籽蛋白质提取率为指标,研究pH值、液料比、时间、温度对蛋白质提取率的影响。结果表明番木瓜籽蛋白质提取率最优条件为:pH 11.3、料液比1∶45(g/mL)、时间224 min、温度47.5℃,针对蛋白质提取率的模型显示失逆项不显著,R2值为94.26%,表明模型拟合良好。在此试验条件下,验证试验结果平均值为53.74%,与理论值55.21%接近。

As a waste and edible papaya seed produced by the industry,it increases its protein extraction rate,realizes the recycling of resources and expands the value of by-products. Using papaya seeds as raw materials,the response surface method was used to optimize the extraction process of papaya seeds by alkali-soluble acid precipitation. The effect of pH,solid-liquid ratio,time and temperature on protein extraction rate was studied by extracting the protein yield of papaya seeds. The results showed that the optimal extraction conditions of papaya seed protein were:pH 11.3,ratio of material to liquid 1 ∶ 45(g/mL),time 224 min,temperature 47.5 ℃. The model for protein extraction rate showed that the irreversible term was not significant,and the R2 value was 94.26 %.,indicating that the model fits well. Under this experimental condition,the average value of the verification test results was 53.74 %,which was close to the theoretical value of 55.21 %.