“ARTP诱变筛选大肠埃希氏菌营养缺陷型”虚拟仿真实验的构建

Design and Manufacture of Virtual Simulation Experiment "Screening for the Nutritional Deficiency Type of Escherichia Coli Induced by ARTP Mutagenesis"

“ARTP诱变筛选大肠埃希氏菌营养缺陷型”虚拟实验教学项目的课程软件基于安全、成熟、易用、高性能、先进的虚拟仿真平台架构进行构建。项目具有“实验内容涵盖诱变育种全周期”“避免不良实验结果影响后续实验”“有效缩短实际实验周期”等优势。虚拟实验过程由“虚拟环境+虚拟仪器+虚拟试剂”构成。实验包含4大模块,诱变处理、营养缺陷型浓缩、营养缺陷型检出、营养缺陷型鉴定以及6个主要操作步骤及29个具体操作步骤。学生可通过自主式、案例式的在线学习,掌握等离子体(ARTP)诱变技术的基本原理;学习营养缺陷型菌株的概念及重要意义;学习了解营养缺陷型菌种筛选、鉴定的原理及常用方法;系统学习、掌握通过ARTP技术诱变大肠杆菌并筛选、鉴定其营养缺陷型的方法及操作等实验目的。

The design and manufacture of virtual simulation experiment"Screening for the nutritional deficiency type of Escherichia coli induced by ARTP mutagenesis"base on safe,mature,easy to use,high performance,advanced virtual simulation platform.The advantages of the project include"the experiment content covers the whole period of mutagenesis breeding","avoid adverse experiment results affecting subsequent experiments",and"effectively shorten the actual experiment period".The process of virtual experiment consists of"virtual environment+virtual instrument+virtual reagent".The experiment consists of four modules:mutagenesis treatment,nutrient deficiency type concentration,nutrient deficiency type detection,and nutrient deficiency type identification,and 6 main operation steps and 29 specific operation steps.Students can master the basic principles of the plasma(ARTP)mutagenesis technology through independent and case-based online learning.To learn the concept and significance of nutritional deficiency strain;to learn the principles and common methods of screening and identification of nutrient deficient strains;to systematically study and master the methods and operation of screening and identifying the nutrition-deficient type of E.coli mutagenesis through ARTP technology.