摘要
绿色荧光蛋白(GFP)作为一种报告基因,具有高灵敏度、稳定性好、低细胞毒害性等优点,被广泛应用在植物与病原菌互作的研究中。文章从GFP转化方法、病原菌侵染寄主过程研究、病原菌数量检测、病原菌亚细胞结构定位研究等方面论述了GFP在植物病原菌研究中的应用情况,为GFP的应用提供参考信息。
As a reporter gene,green fluorescent protein(GFP)has the advantages of high sensitivity,good stability,low cytotoxicity and so on,which has been widely used in the study of the interaction between pathogens and host plants.In this paper,the application of GFP in plant pathogens was discussed from the aspects of transformation method of GFP,the pathogen infection process,the detection of pathogen quantity,and the study on subcellular structure of pathogen.It provided reference information for the application of GFP.
作者
史志丹
宋培玲
郝丽芬
皇甫海燕
燕孟娇
杨永青
郭晨
贾晓清
皇甫九茹
李子钦
张宝辉
陈斐斐
SHI Zhidan;SONG Peiling;HAO Lifen;HUANGFU Haiyan;YAN Mengjiao;YANG Yongqing;GUO Chen;JIA Xiaoqing;HUANGFU Jiuru;LI Ziqin;ZHANG Baohui;CHEN Feifei(College of Life Sciences,Inner Mongolia University,Hohhot 010021,China;Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences,Hohhot 010031,China;Teni River Farm and Pasture of Hulunbuir Nongken Group,Teni River 021024,China)
出处
《北方农业学报》
2019年第6期68-72,共5页
Journal of Northern Agriculture
基金
内蒙古农牧业科技创新基金项目(2017CXJJN15)
内蒙古农牧业青年创新基金项目(2017QNJJNO4,2017QNJJNO2,2018QNJJNO6)。
关键词
绿色荧光蛋白
病原菌
报告基因
Green fluorescent protein
Pathogen
Reporter gene
