空化微射流对生物酶法豆渣蛋白结构影响的拉曼光谱分析

Raman Spectroscopy Analysis of the Effect of Cavitation Microjet on the Structure of Soybean Dreg Protein from Aqueous Enzymatic Extraction of Soybean Oil

为了研究新型物理改性技术--空化微射流对生物酶法制油豆渣蛋白结构的影响,测定了不同空化微射流处理时间(0、5、10、15 min)下豆渣蛋白的拉曼光谱。结果表明:经空化微射流处理的豆渣蛋白中α-螺旋及β-折叠结构相对含量显著增加(P<0.05),β-转角及无规卷曲结构相对含量降低;色氨酸和酪氨酸残基处于"暴露态";二硫键g-g-g和g-g-t模式比例增加,t-g-t模式比例降低。综上所述,空化微射流使豆渣蛋白有序结构增加,提高了其稳定性。本研究结果为生物酶法制油豆渣的开发利用提供一定的参考依据。

In order to explore the effect of cavitation microjet as a new physical modification technology on the structure of soybean dreg protein from aqueous enzymatic extraction of soybean oil, we determined the Raman spectra of soybean dreg protein subjected to different durations of cavitation microfluid treatment(0, 5, 10 and 15 min). The results showed that after cavitation microjet treatment, the proportions of the secondary structure fractions α-helix and β-sheet increased significantly(P < 0.05), and the proportions of β-turn and random coil decreased. The tryptophan and tyrosine residues were in an exposed state;the proportions of disulfide bond modes g-g-g and g-g-t increased while t-g-t decreased. To sum up, cavitation microjet could result in a structural transformation of soybean dreg protein from a disordered to an ordered state, thus improving its stability. This study provides a basis for the development and utilization of soybean dregs from aqueous enzymatic extraction of soybean oil.