摘要
目的:研究添加蛹虫草多糖的益生型酸奶对乙醇所致小鼠肝损伤的保护作用。方法:每日灌胃小鼠体积分数40%乙醇后再灌胃不同蛹虫草多糖添加量(0.03%(低剂量组)、0.06%(中剂量组)、0.12%(高剂量组))的益生型酸奶,共灌胃28 d。测定小鼠的体质量、肝脏指数以及血清、肝脏的生化指标,并取肝脏制作石蜡切片,通过苏木精-伊红染色观察肝组织的病理变化。结果:与酒精性肝损伤模型组小鼠相比,不同蛹虫草多糖添加量的益生型酸奶均可显著降低酒精性肝损伤小鼠的肝脏指数、血清中谷丙转氨酶(alanine aminotransferase,ALT)和谷草转氨酶(aspartate aminotransferase,AST)活性、甘油三酯(triglyceride,TG)、总胆固醇(total cholesterol,TC)以及低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)水平,显著提高血清中高密度脂蛋白胆固醇(high-density lipoprotein cholesterol,HDL-C)水平以及肝脏中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、超氧化物歧化酶(superoxide dismutase,SOD)活力(P<0.05),同时使肝脏丙二醛(malondialdehyde,MDA)水平显著下降(P<0.05)。肝组织病理损伤也得到明显改善。与模型组小鼠相比,高剂量组小鼠肝脏功能提高,血清ALT、AST活力分别降低58.87%、38.89%;肝脏脂肪代谢能力提高,TG、TC、LDL-C水平分别降低39.48%、26.92%、38.54%,HDL-C水平提高20.67%;小鼠肝脏抗氧化能力提高,小鼠肝脏中GSH-Px、SOD活力分别提高53.95%、25.16%,MDA水平降低了49.57%。结论:添加蛹虫草多糖的益生型酸奶对小鼠酒精性肝损伤的保护作用明显强于未添加蛹虫草多糖的酸奶,且添加量越高,对酒精性肝损伤的保护作用越强,添加蛹虫草多糖益生型发酵酸奶可发挥蛹虫草多糖和益生型酸奶在护肝功能方面的协同作用。
Purpose: The protective effects of Cordyceps militaris polysaccharide supplemented probiotic yogurt on alcoholic liver injury in mice were investigated. Methods: Different doses(0.03%, 0.06%, 0.12%) of the probiotic yogurt following 40% ethanol were orally given to mice on a daily basis for 28 days. Body mass, liver index, serum and liver biochemical indicators were determined. Paraffin sections of liver tissue were prepared for observation of histopathological changes by hematoxylin and eosin staining. Results: In addition to remarkably improving liver damage, the probiotic yogurt at all doses could significantly reduce liver index and alanine aminotransferase(ALT) and aspartate aminotransferase(AST) activities as well as total cholesterol(TC), triglyceride(TG) and low-density lipoprotein cholesterol(LDL-C) levels in serum, increase high-density lipoprotein cholesterol(HDL-C) levels in serum and superoxide dismutase(SOD) and glutathione peroxidase(GSH-Px) activities, and decrease malondialdehyde(MDA) level in liver tissue(P < 0.05). Compared with the model group, liver function of mice in the high-dose group was enhanced, and the serum levels of ALT and AST were decreased by 58.87% and 38.89%, respectively. Lipid metabolism in the liver was improved;TG, TC, LDL-C levels were decreased by 39.48%, 26.92% and 38.54%, respectively, and HDL-C level was increased by 20.67%. Liver antioxidant capacity was improved as reflected by the fact that the contents of GSH-Px and SOD in mouse liver were increased by 53.95% and 25.16%, respectively, and MDA content was reduced by 49.57%. Conclusion: The protective effect of probiotic yogurt supplemented with Cordyceps militaris polysaccharides on alcoholic liver injury in mice is significantly higher than that of plain yogurt, which is positively correlated with the concentration of Cordyceps militaris polysaccharides. Therefore, there is a synergistic protective effect of yoghurt and Cordyceps militaris polysaccharides on alcoholic liver injury in mice.
作者
韩瑜玮
刘洋洋
陈伟
HAN Yuwei;LIU Yangyang;CHEN Wei(Key Laboratory of Food Processing Technology and Quality Control in Universities of Shandong Province,School of Food Science and Engineering,Shandong Agricultural University,Tai’an 271000,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第1期209-214,共6页
Food Science
关键词
蛹虫草多糖
酸奶
酒精性肝损伤
协同保护作用
Cordyceps militaris polysaccharide
yogurt
alcoholic liver injury
synergistic protective effect