基于香气活力值的烤烟烟叶清(青)香风格特征分析

Analysis of fresh(green)aroma note of flue-cured tobacco based on odor activity value

为探究不同烤烟烟叶(玉溪C3F和许昌C3F复烤烟叶)的清香风格特征,采用半制备型高效液相色谱(HPLC)和气相色谱-质谱联用(GC-MS)等技术分离鉴定了浓香型和清香型烟叶主流烟气中的香气成分,确定了11种清(青)香成分,通过建立GC-MS内标标准曲线测定了其在烟叶主流烟气中的释放量,计算了相应的香气活力值(OAV),比较了11种清香成分对烟气清香香韵的贡献度,并基于OAV对两种烟叶的清香风格特征进行了分析。结果表明:①2种烟叶主流烟气中11种清香成分的总释放量分别为5.16和4.47μg/支,其中3-乙酰基吡啶和香叶基丙酮的释放量较高;②2种烟叶主流烟气中2-乙基己醇的OAV均小于1,对烟气清香香韵的贡献较小,β-大马酮和二氢大马酮的OAV均较大,在彰显烟气清香香韵方面起主要作用;③除OAV小于1的2-乙基己醇外,其余10种清香成分在玉溪C3F烟叶主流烟气中的总OAV为8.91×106,是许昌C3F烟叶的5倍,这与玉溪C3F烟叶清甜香突出、清香明显的感官评价结果一致。

To develop and enhance characteristics of fresh aroma note of different flue-cured tobaccos(redried leaves of Yuxi C3F and Xuchang C3F),the aroma components in mainstream smoke of flue-cured tobacco of robust and fresh flavor types were separated by semi-preparative high performance liquid chromatography.Eleven fresh(green)aroma components were identified and their releases in mainstream smoke were determined by a GC-MS method with internal standards.The odor activity values(OAVs)of the 11 components were calculated,the contributions of the 11 components for the fresh aroma were compared,and the fresh aroma note of the two types of flue-cured tobacco were analyzed on the basis of the OAVs.The results showed that:1)The total levels of the 11 fresh aroma components in mainstream smoke of Yuxi C3F and Xuchang C3F were 5.16 and 4.47μg/cig respectively,in which the contents of 3-acetylpyridine and geranyl acetone were higher.2)The OAV of 2-ethylhexanol was less than 1,its contribution to the fresh aroma note was minor,while the OAVs ofβ-damascenone and damascene were higher,they played important roles in demonstrating the fresh aroma note of mainstream smoke.3)Besides 2-ethylhexyl alcohol,the total OAV of the other 10 fresh aroma components in mainstream smoke of Yuxi C3F was 8.91×106,five times as that of Xuchang C3F,which confirmed the stylistic characteristic of fresh aroma note was more prominent in Yuxi C3F,it was in accordance with the results of sensory evaluation.