子宫内膜癌组织中DNA错配修复基因表达及意义

Expression and Significance of DNA Mismatch Repair Genes in Endometrial Carcinoma

目的:探究在子宫内膜癌组织中DNA错配修复基因(MMR)的表达情况及其意义。方法:选取2017年1月—2018年12月本院收治的子宫内膜癌患者50例作为内膜癌组,其中包括疑似Lynch内膜癌患者8例,以同期20例内膜癌非典型增生患者作为增生组和子宫内膜正常患者20例作为正常组,比较MMR蛋白在各组中的不同表达情况。结果:三组MMR蛋白表达缺失率分别为36.00%、30.00%和0%,内膜癌组和增生组表达缺失率显著高于正常组(P<0.05);疑似Lynch内膜癌与其他内膜癌患者相比、≤45岁与≥60岁的患者相比,其MMR蛋白表达的缺失率显著较高(P<0.05),内膜癌患者的病理分级则与MMR蛋白的表达无明显相关性(P>0.05);50例内膜癌患者的hMLH1、hMSH2、hPMS2以及hMSH6的表达缺失率分别为24.00%(12/50)、10.00%(5/50)、20.00%(10/50)和8.00%(4/50)。结论:MMR蛋白表达的缺失会导致子宫内膜非典型增生以及内膜癌,特别是低龄内膜癌的出现和进一步发展。

Objective:To investigate the expression of DNA mismatch repair gene(MMR)in endometrial cancer and its significance.Methods:50 patients with endometrial cancer collected in our hospital from January 2017 to December 2018 were selected as the endometrial cancer group,including 8 patients with suspected Lynch endometrial cancer,and 20 patients with atypical hyperplasia of endometrial cancer as the hyperplasia group and 20 patients with normal endometrium as the normal group.The different expressions of MMR protein in each group were compared.Results:The loss rates of MMR protein expression in three groups were 36.00%,30.00%and 0%,respectively.The loss rates of MMR protein expression in endometrial cancer group and hyperplasia group were significantly higher than those in normal group(P<0.05).The loss rates of MMR protein expression in suspected Lynch endometrial cancer patients were significantly higher than those in other endometrial cancer patients(P<0.05).However,there was no significant correlation between the pathological grade and the expression of MMR protein(P>0.05).The expression deletion rates of hMLH1,hMSH2,hPMS2 and hMSH6 in 50 patients with endometrial cancer were 24.00%(12/50),10.00%(5/50),20.00%(10/50)and 8.00%(4/50).Conclusion:The absence of MMR protein expression may lead to atypical endometrial hyperplasia and endometrial cancer,especially in young endometrial cancer.