杨树木屑废弃物酶解液发酵制备富硒酵母的研究

Preparation of selenium-enriched yeast by enzymatic hydrolysis of waste poplar wood chips

以杨树木屑为原料,考察了固液比、酶添加量、pH和酶解时间对其酶解效果的影响。通过优化酶解条件制得葡萄糖质量浓度为55 g/L的酶解液,将酶解液作为碳源替代传统培养基中的葡萄糖,其他营养成分不变,发酵制备富硒酵母,重点研究了酵母菌在酶解液中的生长情况和富硒能力。结果表明,酵母在酶解液培养基中长势良好,5 L发酵罐发酵36 h后,每L发酵液酵母干重23.9 g,单位酵母硒含量超过3500μg/g。

The effects of varying the solid-liquid ratio,enzyme loading,pH and reaction time on the enzymatic hydrolysis of poplar wood were investigated.By optimizing the enzymatic hydrolysis conditions,an enzymatic hydrolysate with a glucose concentration of 55 g/L was obtained.This enzymatic hydrolysate was used as a carbon source to replace glucose in a traditional medium,with the other nutrients unchanged.Selenium-enriched yeast was prepared by fermentation,and the growth of the yeast in the enzymatic hydrolysate and its selenium-enriching ability were studied.The results showed that selenium-enriched yeast grew well in the enzymatic hydrolysate medium.After 36 h of fermentation in a 5 L fermenter,the dry weight of yeast was 24 g/L and the content of yeast selenium exceeded 3500μg/g.