野生种醋栗番茄抗晚疫病资源的筛选与分析

Screening and Analysis of Wild Solanum pimpinellifolium Species Resistant to Late Blight

利用从世界各地收集的182份野生种醋栗番茄(Solanum pimpinellifolium L.),采用喷雾接种法接种我国晚疫病主流生理小种T0,1和T1,2,旨在筛选出抗晚疫病的遗传资源。结果表明,182份种质资源中有25份种质对T0,1小种表现稳定抗性,占总数的13.74%,其中免疫(I)1份,高抗(HR)5份,中抗(MR)19份;对T1,2小种表现抗性的种质资源为22份,占12.09%,其中高抗种质3份,中抗19份。通过分析抗性种质的地理位置,发现抗性种质分布相对集中,主要来源于秘鲁首都利马。利用18个SNP分子标记对抗性种质进行聚类分析,将抗性种质分为两大类群,其中第Ⅰ类群包含13份种质,地理分布相对集中,抗性水平差异较小;第Ⅱ类群包含9份种质,地理分布相对分散,抗性水平差异较大。试验获得的抗晚疫病种质资源,尤其是高抗种质PI390730、LA1604、L03707,可作为优异抗源应用于番茄抗晚疫病育种。

Taking 182 accessions of Solanum pimpinellifolium collected from all over the world,this study inoculated the dominant races T0,1 and T1,2 of Phytophthora infestans in China by spraying inoculation,aiming at screening tomato germplasm resources with resistance to late blight.The results proved that there were 25 accessions out of 182 germplasm resources having stable resistance to race T0,1,accounting for 13.74%of the total materials.Among them,one accession was immune(I),5 accessions were high resistant(HR),and 19 accessions were moderate resistant(MR).There were 22 accessions showing resistance to race T1,2,accounting for 12.09%of the total materials,of which 3 accessions were high resistant and 19 accessions were moderate resistant.Geographic analysis of these resistant germplasms showed that they were located with relative concentration mainly in Lima,the capital of Peru.Clustering analysis on 18 SNP molecular markers showed that accessions with resistance to late blight could be divided into 2 big groups.Group I contained 13 accessions,with relative concentrated geographical distribution and little difference in resistance level.Group II contained 9 accessions,with relative scattered geographical distribution and great difference in resistance level.The obtained germplasm resources with resistance to late blight,especially these with high resistance(PI390730,LA1604,L03707),can be used for breeding tomato resistant to late blight.