护肝益肺方水提物对抗结核药所致肝损伤小鼠保护作用的研究

Study on the protective effect of Hugan Yifei Recipe on anti-tuberculosis-induced liver injury in mice

目的探讨护肝益肺方对抗结核药所致肝损伤小鼠的保护作用。方法将70只小鼠设为空白对照组、模型对照组、阳性对照组、护肝益肺全方低剂量组、全方高剂量组、护肝方低剂量组组、护肝方高剂量组。除空白对照组外,其余组小鼠每天给予100 mg·kg-1·d-1异烟肼+400 mg·kg-1·d-1利福平,空白组给予0.9%氯化钠注射液,阳性对照组给予750 mg/kg水飞蓟宾葡甲胺溶液,全方低剂量组(5 g生药/kg)和全方高剂量组(10 g生药/kg)分别给予护肝益肺全方药,护肝低剂量组(2 g生药/kg)和护肝高剂量组(4 g生药/kg)分别给予护肝方药,连续静脉注射给药2周。末次给药24 h后取材,收集小鼠血液和肝组织样品进行血清中生化指标的检测并观察小鼠肝病理情况。结果连续2周给药利福平、异烟肼,通过生化检测及病理检查证实小鼠肝损伤模型制备成功。护肝益肺全方、护肝方各剂量组能显著抑制肝损伤模型小鼠血清中总胆红素(TBIL)(P<0.01)和碱性磷酸酶(ALP)的升高(P<0.05),亦能抑制肝组织中丙二醛(MDA)的升高(P<0.01),增强肝组织中超氧化物歧化酶(SOD)的活性和谷胱甘肽(GSH)的水平(P<0.05)。病理切片观察也显示护肝益肺全方、护肝方各剂量组较模型组肝细胞脂肪变性明显减轻。结论护肝益肺方水提物对抗结核药所致肝损伤具有明显保护作用,且作用机制与抗氧化作用有关。

Objective To explore the protective effect of Hugan Yifei Recipe on mice with liver injury induced by tuberculosis drugs.Methods Seventy mice were used as blank control group,model control group,positive control group,total square low dose group and high dose group,Baogan square low dose group and high dose group.Except for the blank control group,the other groups of mice were given 100 mg·kg-1·d-1 isoniazid +400 mg·kg-1·d-1 rifampicin daily,the blank group was given 0.9 % saline,and the positive control group was given 750 mg/kg silybin meglumine.The total square low dose group(5 g crude drug/kg)and the high dose group(10 g crude drug/kg)were given to Hugan Yifei whole prescription,Baogan square low dose group(2 g crude drug/kg)and the high dose group(4 g crude drug/kg)was given the liver-protecting prescription,and the ig was administered for 2 weeks.After 24 hours of the last administration,the blood and liver tissue samples of the mice were collected for serum biochemical indicators and the liver pathological conditions of the mice were observed.Results The rifampicin and isoniazid were administered for 2 weeks,and the liver injury model was successfully confirmed by biochemical tests and pathological examination.Hugan Yifei Quanfang and Baoganfang could significantly inhibit the Increase in total bilirubin(TBIL)(P<0.01)and alkaline phosphatase(ALP)(P<0.05)also inhibited the increase of MDA in liver tissue(P<0.05),and enhanced the activity of SOD and the level of GSH in liver tissue(P<0.05 or P<0.01).Pathological section observation also showed that hepatic cell steatosis was significantly reduced in each dose group of Hugan Yifei Quanfang and Hugan Recipe.Conclusion The Hugan Yifei Recipe has obvious protective effect against liver damage caused by tuberculosis drugs,and its mechanism of action is related to antioxidant activity.