四川牡丹花粉生活力测定方法的比较

Comparative Study on Determination Methods of Pollen Viability in Paeonia decomposita

本研究以四川牡丹花粉为材料,利用离体萌发法、亚历山大染色法、TTC染色法及I2-KI染色法,测定四川牡丹花粉的活力。结果表明:开花后第1天花粉即具有活力,第4天花粉活力最高,随着开花时间的推移,花粉活力逐渐下降。开花后1~7 d,用4种测定方法测得的花粉活力变化趋势基本一致。亚历山大染色法与离体萌发法的结果差异极其显著(P<0.01)。TTC染色法和I2-KI染色法,与离体萌发法的结果差异不显著。其中I2-KI测定结果最接近离体萌发法。综合考虑实验条件、实验周期、实验过程和药品保存方法等因素,I2-KI染色法适合四川牡丹野外人工传粉之前快速准确地测定花粉活力。这为探讨四川牡丹濒危的原因、保护种质资源、开展引种驯化和杂交育种提供了理论依据。

In this paper,the pollen viability of Paeonia decomposita was studied using in vitro germination,Alexander staining,TTC staining and I2-KI staining methods.The results showed that the pollen had viability on the first day after flowering,and the highest viability was on the fourth day.As the flowering time lapsed,the pollen viability gradually decreased.The change tendency of pollen viability in 1–7 d after flowering determinated by four methods were basically the same.The results of Alexander staining and in vitro germination were extremely significantly different(P<0.01),while the differences between TTC staining,I2-KI staining and in vitro germination were not significant.The determination results of I2-KI were closer to that of the in vitro germination method.Taking into account factors such as experimental conditions,experimental periods,experimental processes and drug preservation methods,I2-KI staining method was suitable for the rapid and accurate determination of the pollen viability before artificial pollination of P.decomposita in the field.This would provide a theoretical basis for exploring the endangered causes,protecting germplasm resources,introducing domestication and cross breeding of P.decomposita.